Fertilizers and Feeding Stuffs Regulations

Belize Agricultural Health Authority [CAP.211 415 CHAPTER211 FERTILIZERS AND FEEDING STUFFS REGULATIONS ARRANGEMENT OF SECTIONS 1. Short title. 2. Definition. 3. Ingredients of certain articles. 4. Form ofcertificate. 5. Deleterious substances. 6. Disclosure of materials in feeding stuffs. 7. Methods ofanalysis. 8. Use ofadditives. 9. Prohibition. 10. Marking of articles. 11. Posting of samples. 12. Analysis of samaples. 13. Taking of samples, etc .. THE SUBSIDIARY LAWS OF BELIZE [ Prin.ted by the Governm.ent Printer, ] No. I Power Lane, Belmopan, by the authority of the Government of Belize. 9/1979. REVISED EDITION 2003 9/1979 Short title. Definitions. 416 CAP. 211] Belize Agricultural Health Authority 1. 2. CHAPTER211 FERTILIZERS AND FEEDING STUFFS REGULATIONS (Section 14) [10th March, 1979] These Regulations may be cited as the FERTILIZERS AND FEEDING STUFFS REGULATIONS. In these Regulations, unless the context otherwise requires "Act" means the Fertilizers and Feeding Stuffs Act; "amount of protein" means ( a) except in the case of compound feeding stuffs or feed supplements, the amount of nitrogen, other than ammoniacal, nitrate or urea nitrogen, multiplied by 6.25; (b) in the case of compound feeding stuffs or feed supplements, the amount of nitrogen, including urea and ammoniacal nitrogen but not nitrate nitrogen multiplied by 6.25; "antioxidant" means any substance which delays, retards or prevents the development in a feeding stuff or rancidity or other deterioration arising from oxidation; "binder" means any non-nutritional substance which aids the compaction of feeding stuffs; THE SUBSIDIARY LAWS OF BELIZE [ Printed by the Government Printer,] No. 1 Power Lane, REVISED EDITION 2003 Belmopan, by the authority of the Government of Belize. Belize Agricultural Health Authority [CAP.211 "colourant" means any substance, other than a basic feed ingredient, which is added to a feeding stuff only to import colour to a feeding stuff or to an animal product; "compound feeding stuffs" means a product, other than a feed supplement, obtained by mixing two or more materials, including at least one of the materials mentioned in the frrst column of Part II of the First Schedule, to these Regulations for the purpose of this definition the presence of any added substance of a kind referred to in regulation 9 shall be disregarded; "daily ration" means the total quantity offeeding stuffs, expressed on a 12 percent moisture basis, necessary on average for an animal of a given kind: age group and level of production in order to satisfy its nutritional needs; "emulsifier" means any substance, other than a basic feed ingredient, which aids the formation of the unifom1 dispersion of two or more immiscible substances; "feed supplement" means a product obtained by mixing two or more materials, being a product of kind commonly sold or used to supplement other feeding stuffs to an extent ofnot more than one-twentieth of the total quantity; "fibre" means the organic matter calculated as the result of treatment of the feeding stuff according to the method of analysis described in paragraph 7.2 of the Fourth Schedule, to these Regulations; "oil" means the extract obtained as a result of treatment of a feeding stuff according to the method of analysis described in paragraph 3 .21 or 3 .22 of the Fourth Schedule, to these Regulations; "stabiliser" means any substance, other than a basic feed ingredient, which maintains the uniform dispersion of two or more immiscible substances; "sugar" means total reducing sugars after inversion expressed as sucrose; 417 THE SUBSIDIARY LAWS OF BELIZE [ Prin.ted by the Governm.ent Printer, ] No. I Power Lane, REVISED EDITION 2003 Belmopan, by the authority of the Government of Belize. 418 CAP. 211] Belize Agricultural Health Authority Ingredients of certain articles. Form of certificate. Deleterious substances. ''whole feeding stuff' means a mixture of feeding stuffs which by reason of its composition, is sufficient to ensure a daily ration. 3. ( 1) For the purposes of section 4 any article sold under a name set out in Column I of the First Schedule, to these Regulations has the meaning and shall contain the main ingredients set out against that name in Column 2 of that Schedule and the Statutory Statement shall contain inter aliathe percentage of the article mentioned in Column 3 thereof. (2) For the purposes of section 6 the limits of variations permissible in respect of any article named in Column 3 of the First Schedule to these Regulations shall be that set out against that article in Column 4 of that Schedule. 4. The forms of certificate of analysis to be granted by the Analyst shall be as in the Second Schedule to these Regulations. 5. For the purposes of section 12 (1) the following substances are declared to be deleterious substances: ( a) Salts soluble in water, if present in a feeding stuff in proportions likely to be injurious to the health of animals. (b) All substances (poisonous) except those naturally present in the material or materials from which the feeding stuff is derived. (c) Sand, silicious matter or other insoluble matter not naturally associated with ingredients of the feeding stuff which do not fall within the scope of this regulation or which, even if naturally so associated are present in greater proportion than the maximum than maybe expected in average THE SUBSIDIARY LAWS OF BELIZE [ Printed by the Government Printer,] No. 1 Power Lane, REVISED EDITION 2003 Belmopan, by the authority of the Government of Belize. Belize Agricultural Health Authority [CAP.211 419 commercial samples of the feeding material. 6. (1) disclosed. The presence of the following in the feeding stuffs shall be Disclosure of matenalsm feeding stuffs. (a) Husks, chaff, glumes, shudes, hulls, nutshells or skins of nuts, from any source, whether ground or unground, treated or untreated, when used as separate ingredients or artificial mixtures in the manufacture of feeding stuffs. (b) Peat, peamoss, spent or sugar cane pith. (c) Wheat or rye straw. ( d) Sawdust or any other fonn of wood. (2) When the kernels naturally associated in seeds with one or other of the above mentioned under item (a) of paragraph ( 1) of this regulation are present in a feeding stuff along with the materials with which they are so associated, regard shall be had to the proportion of the above materials which might reasonably be expected to accompany such kernels when the seed from which they are derived is in its condition ( natural), provided that feeding in this condition is regarded as a common practice in the feeding oflivestock. 7. The method-; by which analysis offertilizers and feeding stuffs shall be Method of made for the purposes of the Act shall be as set out in the Third and Fourth analysis. Schedules respectively to these Regulations. 8. The use of additives shall be as set out in the Fifth Schedule to these Use of additives. Regulations. 9. ( 1) No person shall sell or have in his possession with a view to Prohibition. sale for use as a feeding stuff or import into Belize for such any material containing THE SUBSIDIARY LAWS OF BELIZE [ Prin.ted by the Governm.ent Printer, ] No. I Power Lane, Belmopan, by the authority of the Government of Belize. REVISED EDITION 2003 420 CAP. 211] Belize Agricultural Health Authority any added antioxidant, colourant, emulsifier, stabiliser, binder, vitamin D2 or D3, copper, cobalt, selenium, manganese, zinc, urea or any added substance of a description specified in the first column of Part V of the table in the Fifth Schedule to these Regulations unless the material complies with the provisions of that Schedule as respects content and, where appropriate marking and it shall be an offence if a sampled portion of any such material does not comply with the provisions of that Schedule as regards content. (2) The provisions of this regulation shall not apply as respects to any antioxidant, colourant, emulsifier, stabiliser, binder, vitamin D2 or D3, copper, cobalt, selenium, manganese, zinc or urea or substance as aforesaid which is (a) for use only in accordance with a prescription given by a veterinary surgeon or practitioner for the treatment of a particular animal or herd under his care; (b) a medicinal product or for use for a medicinal purpose in a feeding stuff; (c) for use only for the purpose of scientific research or experiment and is not generally for sale, purchase or use in a feeding stuff; (d) intended for the exportation to a destination outside Belize and is clearly marked or labelled to that effect. (3) No person shall use as feeding stuff or import into Belize for use any material containing any added substance not being a substance of a name or description specified in any part in the table set out in the Fifth Schedule to these Regulations or in- paragraph 1 (/) or ( g) of that Schedule, which is deleterious to poultry, cattle or to human beings, and it shall be an offence if a THE SUBSIDIARY LAWS OF BELIZE [ Printed by the Government Printer,] No. 1 Power Lane, Belmopan, by the authority of the Government of Belize. REVISED EDITION 2003 Belize Agricultural Health Authority [CAP.211 sampled portion of any such material is shown by analysis of the sample taken from it to contain an added substance which is deleterious as aforesaid. 10. Articles I shall be marked legibly in writing, printing, or stenciling (a) on the article itself or on a label securely attached thereto, or (b) where the article is packed in a number of separate packages either on the wrapper or on the container, or on a label securely attached to each of the packages, or (c) where the article is in a bulk container or tanker (i) on the bulk container or tanker, or a label securely attached thereto, or (ii) where the bulk container or the tanker is a road vehicle, on a document which clearly relates to the material which is retained in the vehicle and is readily available for inspection, or (iii) otherwise in such a manner that the mark shall be readily apparent and unequivocally associated with the material, or ( d) where the article is loose in bags or bales in such a manner that the mark shall be readily apparent and unequivocally associated with the material. 421 Marking of ai1icles. THE SUBSIDIARY LAWS OF BELIZE [ Prin.ted by the Governm.ent Printer, ] No. I Power Lane, REVISED EDITION 2003 Belmopan, by the authority of the Government of Belize. 422 CAP. 211] Belize Agricultural Health Authority Posting of samples. Analysis of samples. Taking of samples, etc .. 11. Any part of a sample required to be sent to any person shall be sent by registered post. 12. Where a sample ofa feeding stuff has been taken by an inspector in the prescribed manner and sent to an agricultural analyst for analysis, any such analysis of the oil content shall be disregarded unless it is carried out before the end of three weeks commencing the date of sampling. 13. The mannerof taking, dividing, marking, sealing and fastening of samples shall be as described in the Sixth Schedule to these Regulations. THE SUBSIDIARY LAWS OF BELIZE [ Printed by the Government Printer,] No. 1 Power Lane, REVISED EDITION 2003 Belmopan, by the authority of the Government of Belize. o:i (I) ::,-" (I) 3 "" ::,. ::;. (I) 0.. o.g z cr o ~ O -< < "' . ~ u >-' g:. ~ ~ '""C(D ~ ,-+ ~ 0 :j ;:.-' < 0 ,-+ (D (D < >-\ (I) 8,~r-8 o:i 8'- ~ s (D O (D (D ~ ~-- u a (I) '< "" . 0 :::. ..., ::;. ,fl ""' = i:"l [I) t'.: = [I) s > ~ t"' ~ [I) 0 "'l = i:"l t"' ~ ~ ;:; [I) i:"l 1::1 i:"l 1::1 :::; 0 2 N = = '-' Column I Name ofartid: Compound fertilizer Ammonium nitrate Ammonium suphate nitrate Calcium cyanarnide Calcium nitrate Nitrogenous gas liquor, anmmoniacal gas liquur, gas liquor Arrm1oniun1 s11phate Urea Basic slag FIRST SCHEDULE PART I FERTILIZERS SECTION 4 (a), (b) AND (C), AND SECTION 6 (I b) Column H Column HI Meanings/Ingredients of articles Ingredients the percentage of which are to be declared in the statutory statem:nt A rnixture of any two or more of Amounts, fany of nitrogen, potash the materials mentioned in this phosphoric acid table Ammonium nitrate for ferti]izjng Amount of nitrogen purposes A mils.iure ofammoniwn sulphate A1mLU)t of nitrogen and nitrate Cornmercid calcium cyanarnide Anx:iunt of nitrogen Calcium nitrate for fertilizing A1mtmt of nitrogen pw-poses Ammonia.cal liquor produced in Amount ofnitrogen the carbonsation of coal. free from visible tar Ammonium sulphate for ferti]izjng A1munt of nitrogen purposes Cornmercidly pur, tu-ea containing Ammmt ofnitrogen less than l .5 % b iun::t A by-prodxt containing Total amount of phosphoric acid phosphoro .is, obtained in the manufactur:: of stgeeal to which no addition haJ been made Column IV Limits of\1iriatio1 Nitrogen, potash and phosphoric acid l O~ic, 5% of the amourt stated 5% of the amoLU1 stated 5% of the arnom1 stated 5% of the arnom1 stated 5% of the arnour:t stated 5% of the amourt stated 5% of the amoUJ1 stated 10% of the aimunt stated ~ (1-) ~ (1-) ~ ~ r')" :;: ~ 2; ... ~ §: ~ :;: ~ C) "'I ~ ?S > :cl N ..... ..... +" N w "" ::,. 0:: ::;. (!; (!; ~ 3 0 ~~ ~~ < :j . (I) - -- ~ I:)" § ~ ""(!; " SC~ ~ g (D (D < >-l (I) 8,~r-8 o:: SC ~ S (D O (D (D ~ ~-- ~ a ~ '-< ~ 0 -· .., ::;. ,fl ""' = i:"l [I) t'.: = [I) s ~ t"' ~ [I) 0 "'l = i:"l t"' N i:"l :.i i:"l ;:; [I) b i:"l 1::1 :::; 0 2 N = = '-' Column [ DicalciLm1 phosphate Phosphate rock, ground or othenvise Superp hosphate Superphospbate concentrated Superphosphate, triple Kainit Magnesium Kainit Potassium chloride Potassium sulphate FIRST SCHEDULE (cont.) Column U Column DI Dicalcium phosphate for fertilizing /\rnoimt of phosphoric acid pmpmes The sTibstance obtained from Amount of phosphoric acid minienl deposits, to which nothing else has been added Phosphate rock treated with Amount of phosphoric acid sulphuric acid ( 1) Prosphate rock treated with sulphuric acid and phosphoric acid. ('.::) Ptosphate rock treated with phosphoric acid only. (I) Amow1t of phosphoric acid C:!> Amount of phosphoric acid A mireral pofassium salt with or Amount of potash without magnesirnn Minenl postassitrn salt Amount of potash contahing at least 3.6% n1agncsium Potassirnn chloride for :rertilizi.ng i\mount of potash purpme Potassium chloride fi.Jr J:ertilizing AmOlmt of potash purpme Column IV 5% of the an.101.mt stated 10% of the amount stated l 0'% of the amount stated 1 O'?/, of the amoimt stated 10% ofthe amount stated 5% of the a1110i.mt st1ted 5% of the an1<)1.rnt stated 5% of the amount stated 5% of the ammmt stated +" N +" (j > :-= N ..... = ~ (1-) ~ (1-) I~ r')" :;: ... :2" 2; ... ~ l::l §: ~ :;: ~ a '"I 1~· "" ::,. to a - ,., ,., ::,-" - 0.. ,., 3 o.g z cr o ~ O -< < "' . ~ u >-' g:. ~ ~ '""C(D ~ ,-+ ~ 0 :j ;:.-' < 0 ,-+ (D (D < >-\,., 8,~r-8 o:i 8'- ~ s (D O (D (D ~ ~-- u a ,., '< "" . 0 :::. ..., ~ ,fl ""' = i:"l [I) t'.: = [I) s > ~ t"' ~ [I) 0 "'l = i:"l t"' ~ ~ ;:; [I) i:"l 1::1 i:"l 1::1 :::; 0 2 N = = '-' Coht11m I Potassium nirate Potassic nitrate of soda Chilean potash nitrate Potassic basic Bone meal, obtained by grinding or otherwise treating bone Dried blood Fish residues Hoo:fu Hoofs and h:ims Horns Meat and bone residue FIRST SCHEDULE (cont.) Cohmm II Colullill III Potassium nitrate tor Arnmmts of nitrogen and potash fertilizill; purposes A mixture of sodium and AnxJtmts of nitrogen and potash , nitrates For forilizing pmposes Amounts of nitrogen and potash A mixture of basic slag and Am.:mnt<; of phosphoric acid potassium chloride or Amount of potash sulphate Commucially pure bone, Amounts of nitrogen and raw, degreased or steamed, phosphori:: acid ofwhic1 90% passes a sieve of 1 /4 i:lch square aperture A product dried and ground Amornlt of nitrogen to which no other matter has been added. A product dried and ground Amounts of nitrogen and to which no other matter has acid been added A product, crushed and l\mount of nitrogen ground to which no other matter has been added A mL\.iure of hoof and horn, Amount of nitrogen crushed or ground to which no other rnatter has been added A product, crushed and i\.iuountofnitrogen grmmd to which no other matter has been added A prodllct of drying and Amo1mt of nitrogen and phosphoric grinding bone, flesh and acid other shughterhouse residues Colillllll IV 5% ofthe amount stated 5%, of the amount stated 5% of the amount stated 10% of the arnourt stated 10% of the amour t stated l 0% of the amourt stated I 0% of the amour:t stated 10% of the amorn~t stated 10% of the amourt stated 10% of the amornt stated I 0% of the amourt stated ~ (1-) ~ (1-) ~ ~ r')" :;: ~ 2; ... ~ §: ~ :;: ~ C) "'I ~ ?S > :cl N ..... ..... +" N V, "" ::,. 0:: ::;. (!; (!; ~ 3 0 ~~ ~~ < :j . (I) - -- ~ I:)" § ~ ""(!; " SC~ ~ g (D (D < >-l (I) 8,~r-8 o:: SC ~ S (D O (D (D ~ ~-- ~ a ~ '-< ~ 0 -· .., ::;. ,fl ""' = i:"l [I) t'.: = [I) s ~ t"' ~ [I) 0 "'l = i:"l t"' N i:"l :.i i:"l ;:; [I) b i:"l 1::1 :::; 0 2 N = = '-' Column l Oil seed furtili7 ers, obtained by rernoval of oil from seeds Column l N arne o farticle Compound foeding stuff Artificially dred grass, any other artificially dried green crop o: a mixture ofany of them Coconut or copra meal or cake FIRST SCHEDULE (cont.) Column II Column HI The residue obtained the Anxnmt v".,,.,~e-" rc1rnval of oil from conm-iercia!Jy pure seed PART II FEEDING STUFFS Column U Column m Meaning/Ingredients of Ingredients the percentages of which are articles to be declared in the statutory statement A mixtru:e of any two or more Ammmt, ifany, of protein (stating as of the materials mentioned in included therein the ammmt, this table of protein equivalent of urea and ammonia) wd ammmt, ifany of oil and fibre product vYhether ground Amount of protein or otherwise which (a) is obtained be artificially drying gr..1ss, green cereal or mixture of them 1b) has had no other substanc: added thereto The residue resulting from the Amount ofoil and protein respectiYely remnal of oil from commercially pure nut Column IV l 0% of the amount stated Column IV Liniits of variation JO% of the amount stated in each case JO% of the am)unt stated in each case l 0% of the a1rnunt stated in rnch case +" N °' (j > :-= N ..... = ~ (1-) ~ (1-) I~ r')" :;: ... :2" 2; ... ~ l::l §: ~ :;: ~ a "'I I~ "" ::,. to a - ,., ,., ::,-" - 0.. ,., 3 o.g z cr o ~ O -< < "' . ~ u >-' g:. ~ ~ '""C(D ~ ,-+ ~ 0 :j ;:.-' < 0 ,-+ (D (D < >-\,., 8,~r-8 o:i 8'- ~ s (D O (D (D ~ ~-- u a ,., '< "" . 0 :::. ..., ~ ,fl ""' = i:"l [I) t'.: = [I) s > ~ t"' ~ [I) 0 "'l = i:"l t"' ~ ~ ;:; [I) i:"l 1::1 i:"l 1::1 :::; 0 2 N = = '-' Column I Cotton cakes or meals not decorticated Cotton cakes or meal;;, not decorticated or partly decorticated coton seed Dried brevvery Dried distillery products ( other than yeast and matt Dried yeast FIRST SCHEDULE PART II ( cont.) Column II Column rn The residue resulting from the Am.mnt of oil and protein respectively removal of oil from commercially pure cotton not decorticated The residue resulting from the AJIDunt of oiL protein and fibre removal of oil from commercially respectiy;;ly pure cotton seed from which the ~ortex. in whole or part, has been removed The material produced by drying Amrnnt of oil and protein respectively the residue )f rnatlted and unniaked ~ereals usec in brewing, to which no other n12tter has been added Almunt of oil and protein respecfo·ely, of fibre if present in excess of2% and calchim if present in excess of2% A material produced by drying Am.mnt of protein yeast residres to which no other matter has been added Colunm IV 10% of the anount stated in each case 10% of the a1:1ount stated in each case 10% of the ,mount stated in each case 10% of the anount stated in each case l 0% of the anount stated ~ (1-) ~ (1-) ~ ~ r')" :;: ~ 2; ... ~ §: ~ :;: ~ C) "'I ~ ?S > :cl N ..... ..... +" N -..., "" ::,. 0:: ::;. (!; (!; ~ 3 0 ~~ ~~ < :j . (I) - -- ~ I:)" § ~ ""(!; " SC~ ~ g (D (D < >-l (I) 8,~r-8 o:: SC ~ S (D O (D (D ~ ~-- ~ a ~ '-< ~ 0 -· .., ::;. ,fl ""' = i:"l [I) t'.: = [I) s ~ t"' ~ [I) 0 "'l = i:"l t"' N i:"l :.i i:"l ;:; [I) b i:"l 1::1 :::; 0 2 N = = '-' Column 1 Feed supplement Feeding bone flour or any other bone product for feeding purposes Feeding dried blood Feeding meat or bone meal FIRST SCHEDULE PART Il ( cont.) ColumnU Column Ill Instructions fur mixing with other feeding stuffs or infonnation as to use vYhere is red direct tc animals. Protein equivalent of urea, if any Commercially pur bone, raw or Amounts of phosphoric acid and degreased from which nitrogen protein oay haYe been partially emoved by steam Blood which has been dried to Amounts of nitrogen which no oth:r matter has been added The product containing not less Amounts of oil, protein and than 40% protein and not more phosphoric acid 4% salt obtaned by drying and grinding anirraJ carcasses or 1:ortions thereof ( excluding hoof, horn and feathers) to which no other matter us been added but which may ha,e been ~reliminarily 1reated for the rc1roval of fat ColumnIY Protein ofuea " iodine. cobalt, copper. iron, manganese, zinc, molybdenum, selenium, vitamin D2 and D3 30% or amount stated. Other vitamins no upper :inrit, ifless, 30% of the a1munt stated JO% of the amount s1Lted in each case 10% of the arrount stcted 10% of the amotmt ststed in each case +" N oc (j > :-= N ..... = ~ (1-) ~ (1-) I~ rS· :;: ... :2" 2; ... ~ l::l §: ~ :;: ~ a "-I 1~· "" ::,. to a - ,., ,., ::,-" - 0.. ,., 3 o.g z cr o ~ O -< < "' . ~ u >-' g:. ~ ~ '""C(D ~ ,-+ ~ 0 :j ;:.-' < 0 ,-+ (D (D < >-\,., 8,~r-8 o:i 8'- ~ s (D O (D (D ~ ~-- u a ,., '< "" . 0 :::. ..., ~ ,fl ""' = i:"l [I) t'.: = [I) s > ~ t"' ~ [I) 0 "'l = i:"l t"' ~ ~ ;:; [I) i:"l 1::1 i:"l 1::1 :::; 0 2 N = = '-' Column I Feeding meat ~al Fish meal, white fish meal Maize, flaked Maize germ cake or meal FIRST SCHEDULE PART 11 (cont.) Column II Column III lli product, containing not less Amounts of oil, protein and than 55% prctein and not more phosphorc acid salt obtahed by drying and grinding animal carcasses or portions thereof ( excluding hoof, horn and foathers) to which no other matter has been added but which may have been preliminarily treated for the removal of fat A product oliained by drying and Amounts of oil, protein phosphoric grhding or othe1wise treating fish and salt or waste of fish, to which no other matter has been added A product obtained by cooking Amounts of oil and protein anf flaking commercially pure 1mi?.e, either as grown, or frorna whch the ge1m in whole or part, has been rernDved A meal or cake resulting from the Amounts of oil and protein grhding of maize germs or from which oil 1as been removed in wlole or in part Column IV lOo/, of the annllilt stated in each case l Oo/t of the anmmt stated in each case 10¾ of the mmllilt stated in each case 10¾ of the mmunt stated in each case ~ (1-) ~ (1-) ~ ~ r')" :;: ~ 2; ... ~ §: ~ :;: ~ C) "'I ~ ?S > :cl N ..... ..... +" N 'D "" ::,. 0:: ::;. (!; (!; ~ 3 0 ~~ ~~ < :j . (I) - -- ~ I:)" § ~ ""(!; " SC~ ~ g (D (D < >-l (I) 8,~r-8 o:: SC ~ S (D O (D (D ~ ~-- ~ a ~ '-< ~ 0 -· .., ::;. ,fl ""' = i:"l [I) t'.: = [I) s ~ t"' ~ [I) 0 "'l = i:"l t"' N i:"l :.i i:"l ;:; [I) b i:"l 1::1 :::; 0 2 N = = '-' Column. I Maize gluten fe~d Maize meal Milk powders, including oil and/ or fat fortified milk powders Mixture of mo hisses and urea Molasses fueds Oil cakes or meals which are the product of undecorticated material from which oil has been removed FIRST SCHEDULE PART II ( cont.) Column II ColmmIII A by-product resuking from the Amoun1s of oil and protein removal of starch and genn from maize, to which no other matter has been added The meal obtained by grinding Amoun1s of oil and protein commercially pure as grown The produ::t obtained by drying Amounts of oil and protein milk from which oil may be have been removed or added SU!,rar and protein equivalent of urea Any mixture, other than molasses Am)un1s of sugar and fibre and urea, containing not less than 10% sugar or an absorbent material treacle or molasses The produ::t obtained from Ammmt of oil and protein commercially pure nuts or beans Column IV 10% of the amount stated in e2.ch case 10% of the ammint stated in ec:ch case l 0% of the amount stated in each case 10% of the arnmmt stated in each case 10% of the ammm.t stated in each case l 0% of the ammrnt stated m case +" w 0 (j > :-= N ..... = ~ (1-) ~ (1-) I~ r')" :;: ... :2" 2; ... ~ l::l §: ~ :;: ~ a "-I I~ "" ::,. to a - ,., ,., ::,-" - 0.. ,., 3 o.g z cr o ~ O -< < "' . ~ u >-' g:. ~ ~ '""C(D ~ ,-+ ~ 0 :j ;:.-' < 0 ,-+ (D (D < >-\,., 8,~r-8 o:i 8'- ~ s (D O (D (D ~ ~-- u a ,., '< "" . 0 :::. ..., ~ ,fl ""' = i:"l [I) t'.: = [I) s > ~ t"' ~ [I) 0 "'l = i:"l t"' ~ ~ ;:; [I) i:"l 1::1 i:"l 1::1 :::; 0 2 N = = '-' Column I Oil cakes or meals which are the product of any one decorticated or partly deccrticated 1mterial from which oil has been removed Rice bean or meal Nut cakes or meals Wheat o:ffuls or millers offals FIRST SCHEDULE PART II ( cont.) Colmm1H Colu.nm HI TI1e product obtained from Amount of oil and protein commercially pure nuts or beans TI1eby-productproducedin .Arnmnt of oil, protein and milling srelled rice to which nc hbre other ma:ter has been added The residue resulting from the Amount of oil and protein removal ,Jf oil from commercially pure nut kernels A product of wheat separated Amount of fibre in the process of milling and containing not more than 4% of vegetable matter, other than ex.tracted from wheat h the process of cleaning by the maker of the o:ffuls in the production of flour Column IV 10% of the amount stated in e2.ch case 10% of the amount stated in e;:ch case 10% ofthe am)unt stated in Cicch case Not to exceed 10% of the ammnt staled ~ (1-) ~ (1-) ~ ~ r')" :;: ~ 2; ... ~ §: ~ :;: ~ C) "'I ~ ?S > :cl N ..... ..... +" w 432 CAP. 211] Belize Agricultural Health Authority SECOND SCHEDULE FORM I FQRM OF CERTIFICATE OF ANALYSIS (Section JO) CERTIFICATE OF ANALYSIS OF FERTILIZER (1) I, the undersigned, agricultural analyst for the Belize Government, in pursuance of the provisions of the hereby certify that I received on the day of 20 , from(2) one part of a sample for amdysis; which was duly sealed and and was accompanied , as follows: ( 6) of(3) fastened up and marked ( 4) by a (5) and also by a signed statement that the sample was taken in the prescribed manner; and that the said part has been analysed by me, or under my direction, and I declare the results of analysis to be as follows: (7) % ppm Nitrogen (N)............................. Boron (B) ............................ . Phosphoric acid(P 2 0 5 )Total .... Cobalt (Co) ......................... . Potash (K 2 0) ........................... Copper (Cu) ...................... .. Iron (Fe) .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. . Manganese (Mn) ................. . Magnesium (Mg) .. ...... .. ...... .. .. Molybdenum (Mo) .............. . Neutralising value expressed in terms of calcium oxide . . . . . . . . . . . . . . . . . . . . . . . % Names of herbicides and pesticides found % and I am of opinion that (8) ...................................................................... .. The analysis was made in accordance with the Fertilizers and Feeding Stuffs Regulations. As witness my hand this day of 20 THE SUBSIDIARY LAWS OF BELIZE (Signature and address of analyst) [ Printed by the Government Printer,] No. 1 Power Lane, Belmopan, by the authority of the Government of Belize. REVISED EDITION 2003 Belize Agricultural Health Authority [CAP.211 SECOND SCHEDULE FORM 1 (coNr.} (1) Statements made in the certificate are to be confined to matters which are necessary to venfy compliance with the Act. (2) Here insert the name of the inspector who submitted the sample for analysis; and also the mode of transit, i.e. "by hand", "by registered post", etc. as the case may be. (3) Here insert the name or description applied to the material. ( 4) Here insert the distinguishing mark on the sample. ( 5) Here insert either "statutory statement", "copy of statutory statement", "copy of particulars marked on the material", or "copy of particulars indicated by a mark on or indicated by a mark applied to the material", as the case may be. ( 6) Here insert the particulars contained in the statutory statement, or particulars marked on or indicated by a mark applied to the material, or as the case maybe. (7) Insert relevant results under the appropriate headings, i.e. percentage or parts per million. (8) Here enter intormation as tallows: (a) If the material was sold under a name mentioned in the first column of Part I of the First Schedule, state whether it accords with the meaning given in the second column; and if not, in what respect. (b) If the composition of the material agrees with or does not differ by more than the limits of variation from the statement of particulars contained in the statutory statement, or the particulars marked on or indicated by a mark associated with the material, state that the particulars are correct within the limits of variation. ( c) If the composition of the material diners by more than the limits of variation from the particulars contained in the statutory statement, or the particulars marked on or indicated by a mark associated with the material, state the diflerence between the amount found and the amount stated, and that the difference is outside the limits of variation; and that the difference is to the prejudice of the purchaser, if such is believed to be the case. 433 THE SUBSIDIARY LAWS OF BELIZE [ Prin.ted by the Governm.ent Printer, ] No. I Power Lane, REVISED EDITION 2003 Belmopan, by the authority of the Government of Belize. 434 CAP. 211] Belize Agricultural Health Authority (These notes and the numbers referring to them are for guidance only and do not form part of the certificate.) FORM II CERTIFICATE OF ANALYSIS OF FEEDING STUFF (1) I, the undersigned, agricultural analyst for the Belize Government, in pursuance of the provisions of the , hereby certify that I received on the day of , 20 from (2) one part of a sample of (3) for analysis; which was duly sealed and fastened up and marked ( 4) and was accompanied by a ( 5) as follows: (6) and also by a signed statement that the sample was taken in the prescribed manner; and that the said part has been analysed by me or under my direction, and I declare the results ofanalysis to be as follows: (7) % ppm Oil .......................................... . Protein: Total, including ........... . Protein equivalent of urea ....... . Protein equivalent of urea Fibre ...................................... . Sugar ..................................... . Salt (NaCl) .......................... .. Phosphoric acid(P 2 0 5 ) .......... . Calcium (Ca) ........................ .. Copper (Cu) ......................... . Magnesium (Mg) .................. .. units/kg or IU/kg VitaminA ................................... . Vitan1in D2 ................................ .. Vitan1in D3 ................................. . VitaminE ...................................... . Other vitamins or pro ..................... . Vitan1ins ........................................ .. Pennitted antioxidant (8) .............. .. Permitted colourant (8) ................ .. (9) ................................................. .. (10) .............................................. . THE SUBSIDIARY LAWS OF BELIZE [ Printed by the Government Printer,] No. 1 Power Lane, Belmopan, by the authority of the Government of Belize. REVISED EDITION 2003 Belize Agricultural Health Authority [CAP.211 Molybdenum (Mo) ........................ .. Selenium (Se) ................................ .. Iron (Fe) ......................................... . Iodine (I) ........................................ . Cobalt (Co) .................................... . Manganese (Mn) ............................ . Zinc (Zn) ........................................ . ( 11) Analysis for oil was completed on and! am of opinion that (12) The analysis was made in accordance with the Fertilizers and Feeding Stuffs Regulations. As witness by my hand this day of 20 (Signature and address of analyst) ( 1) Statements made in the certificate are to be confined to matters which are necessary to verify compliance with the Act. (2) Here insert the name of the inspector who submitted the sample for analysis; and also the mode of transit, i.e." by hand by registered post or as the case maybe. (3) Here insert the name or description applied to the material. (4) Here insert the distinguishing mark on the sample and the date of sampling shown thereon. ( 5) Here insert either "statutory statement", "copy of statutory statement", "copy of particulars marked on the material" or "copy of particulars indicated by a mark applied to the material", or as the case may be. ( 6) Here insert the particulars contained in the statutory statement, or particulars marked on or indicated by a mark applied to the material, or as the case maybe. (7) Insert relevant results under the appropriate headings, i.e. percentage, parts per million orunits/kg or IU/kg. (8) Here indicate whether the antioxidant or colourant is an antioxidant listed in Part I of the table to the Fifth Schedule or a colourant listed in 435 THE SUBSIDIARY LAWS OF BELIZE [ Prin.ted by the Governm.ent Printer, ] No. I Power Lane, REVISED EDITION 2003 Belmopan, by the authority of the Government of Belize. 436 CAP. 211] Belize Agricultural Health Authority SECOND SCHEDULE FORM 11 (cont.) Part II of the table to the Fifth Schedule. (9) Here md1cate the presence of any emulsifier, stab1hser or bmder not listed in Part III of the table to the F(fth Schedule. ( 10) Here insert the name and estimated percentage of any ingredient found in the sample, being an ingredient deleterious to animals of any description or to human beings. (11) In the case of a sample of any feeding stuff containing oil insert the date of completion of the oil analysis. (12) Here enter information as follows: (a) If the material was sold under a name mentioned in the first column of Part II, state whether it accords with meaning given in the second column; and if not, in what respect. ( b) lt the composlt1on ot the matenal agrees with or does not dltler by more than the limits of variation from the statement of particulars contained in the statutory statement, or the particulars marked on -or indicated by a mark associated with the material, state that the particulars are correct within the limits of variation. ( c) If the composition oft he material differs by more than the limits of variation from the statement of particulars contained in the statutory statement, or the particulars marked on or indicated by a mark associated with the material, or as the case may be, state the difference between the amount tound and the amount stated, and that the difference is outside the limits of variation, and that the difference is to the prejudice of the purchaser if such is believed to be the case. ( d) lfthe matenal 1s not smtable tor use as a teedmg stuff havmg regard to the Act, state in what respect. ( l hese notes and the numbers retemng to them are tor gmdance only and do not form part of the certificate.) THE SUBSIDIARY LAWS OF BELIZE [ Printed by the Government Printer,] No. 1 Power Lane, Belmopan, by the authority of the Government of Belize. REVISED EDITION 2003 Belize Agricultural Health Authority [CAP.211 THIRD SCHEDULE METHODS OF ANALYSIS OF FERTILIZERS (In this Schedule a "decimal" system had been adopted for the numbering of divisions and sub-divisions. Main divisions are given numbers which precede a decimal puinl. Each ~ub-Ji vbiun inlu which a main Jivbiun b fir~l Ji viueu b distinguished by a digit immediately following the decimal point. For example, the main division 5 is divided into three sub-divisions numbered 5 .1, 5 .2, and 5 .3 respectively. Succeeding digits indicate further sub-division with the result that, for example, the sub-division numbered 5 .1 may itself be divided into sub-divisions numbered 5 .11, 5 .12, 5.13 etc. and those sub-divisions may be further divided in the same way (thus, 5. 111, 5.112, 5.113 etc.) and so on. The main Divisions in the Schedule are as follows: 1. Preparation of the sample for analysis. 2. Determination of moisture. 3. Delerrninaliun ufNilrugen. 4. Determination of Phosphoric acid. 5. Determination of Potash. 6. Determination of neutralising value in liming materials. 7. Determination of magnesium in lime and ground limestone. 8. Determination ofboron. 9. Deltarninaliun ufcubull. 10. Detennination of copper. 11. Detennination ofiron. 12. Delerrninaliunufmagne~ium. 13. Determination of manganese, 14. Determination of molybdenum. NOTE: Reference to "water'' mean distilled water. All reagents used should be of analytical quality. 437 THE SUBSIDIARY LAWS OF BELIZE [ Prin.ted by the Governm.ent Printer, ] No. I Power Lane, REVISED EDITION 2003 Belmopan, by the authority of the Government of Belize. 438 CAP. 211] Belize Agricultural Health Authority 1. Preparation of the sample for analysis With some materials, fine grinding may lead to loss or gain of moisture, and allowance for this may be made. Grinding should be as rapid as possible and unnecessary exposure to the atmosphere avoided. Grinding in a laboratory mill is usually quicker than grinding in a mortar although the latter is permissible. 1.1 Procedure For solid fertilizers, weigh the whole sample and then empty on to a smooth dry surface. Remove, and allow for in the calculation of results, any obvious extraneous matter e.g. metallic particles which may be present in samples of basic slag. 1.11 Dry Powdered and Granulated Fertilizers Grind the sample as finely as possible. Mix thoroughly and take a representative portion of about 250 g. Transfer to a non-corrodible container provided with an air-tight closure. 1.12 Crystalline Fertilizers. e.g. Sulphate of potash and Nitrate of Soda Grind the sample as finely as possible. Mix, withdraw a portion for analysis and transfer to a non-corrodible container provided with an airtight closure. 1.13 Hoof Meal In the case of hard samples of hoof meal which cannot be ground in the "as received" condition, determine the moisture in the sample by the method described in paragraph 2. Then grind the dried portion in a mill and transfer to a non-corrodible container provided with an air-tight closure. Determine the moisture in this prepared sample and calculate the result of analysis of this sample to the "as received" condition. THE SUBSIDIARY LAWS OF BELIZE [ Printed by the Government Printer,] No. 1 Power Lane, Belmopan, by the authority of the Government of Belize. REVISED EDITION 2003 Belize Agricultural Health Authority [CAP.211 1.14 Fertilizers in a Moist Condition Mix the sample well and withdraw a portion for moisture determination. Determine the moisture in this portion by the method described in paragraph 2. (In the case of fertilizers in which ammonia is lost on heating or of fertilizers containing soluble phosphoric acid, the sample should be dried either by placing it in a desiccator over calcium chloride or silica gel, or alternatively by passing dry air at room temperature over the sample until it is in a suitable condition for grinding and sieving). For subsequent analysis, dry a further portion under similar conditions and grind this dried portion in a mortar or mill. Mix thoroughly and transfer to a non-corrodible container provided with an air-tight closure. Determine the moisture in a portion of this prepared sample. Calculate the results of analysis of the sample to the "as received" condition. 1.13 Liquid Fertilizers Shake to mix thoroughly, ensuring that any insoluble matter is thoroughly dispersed immediately before drawing a portion of the sample for analysis. 2. Determination of Moisture Weigh to the nearest mg. about 5 g. of the sample, heat at lO00C for two to three hours, cool in a desiccator and weigh. Reheat for another hour, cool and reweigh. If the difference in weight exceeds 10 mg, continue the heating and cooling procedure until a weight constant within 2 mg is attained. Calculate the total loss of weight as a percentage of the original weight and regard as moisture. 3. Determination of Nitrogen The relevant methods of analysis arc described in the following paragraphs. 3 .3 Total nitrogen ( organic and ammoniacal) in the absence of nitrates. 3 .4 Total nitrogen ( organic, ammoniacal and nitrate) in the presence of nitrates. 439 THE SUBSIDIARY LAWS OF BELIZE [ Prin.ted by the Governm.ent Printer, ] No. I Power Lane, REVISED EDITION 2003 Belmopan, by the authority of the Government of Belize. 440 CAP. 211] Belize Agricultural Health Authority 3.1 Reagents Aluminium ammonium sulphate. Devarda 's alloy - finely powdered- not less than 80% to pass through a sieve having apertures of about 0.25 mm square. Indigo carmine standard solution -Cautiously add 40 ml concentrated sulphuric acid to 1 g indigo carmine (B.P. quality) and stir until dissolved. Pour the solution into 800 ml water, cool and dilute to 1 litre. Adjust the strength of the solution to comply with the following test: Add 20 ml to a solution of 4 mg potassium nitrate in 20 ml water. Add rapidly 40 ml concentrated sulphuric acid and heat to boiling point; the blue colour is just discharged in one minute. Boric acid Indicator Solution -Add 5 ml of indicator solution (0.1 % methyl red and 0.2% bromocresol green in alcohol) to 1 litre saturated boric acid solution. Paraffin Wax. Sodium sulphate or potassium sulphate - anhydrous. Sodium hydroxide solution, 5% w/v - Dissolve 50 g sodium hydroxide in water and dilute to 1 litre. Sodium hydroxide solution, 50% w/v - Dissolve 500 g sodium hydroxide in water and dilute to 1 litre. Sulphuric acid, concentrated ( d = 1.84)- Nitrogen free. Sulphuric acid, 10% v/v -To 500 ml water cautiously add 100 ml concentrated sulphuric acid. Cool and dilute to 1 litre. Sulphuric acid, 50% v/v -To 500 ml water cautiously add 500 ml concentrated sulphuric acid. Cool and dilute to 1 litre. Sulphuric acid ( or hydrochloric acid), 0.2 N. THE SUBSIDIARY LAWS OF BELIZE [ Printed by the Government Printer,] No. 1 Power Lane, Belmopan, by the authority of the Government of Belize. REVISED EDITION 2003 Belize Agricultural Health Authority [CAP.211 3.2 Test for absence of Nitrates Shake 5 gofthe samplewith80ml water in a 100ml volumetric flask. Add 1 g almmmum ammomum sulphate, dllute to l UU ml. Shake well and filter mto a dry beaker. Dilute 1 ml of the filtrate with 8 ml water. Add 1 ml indigo cannine solution and 10 ml concentrated sulphuric acid. Heat to boiling point. If blue colour is not discharged, regard the sample as free from nitrates. 3.3 Total Nitrogen (organic and ammoniacal) in the absence of Nitrates 3 .31 Weigh to the nearest mg. about 2 g of the sample ( or such an amount as shall contain not more than 250 mg nitrogen) and transfer to a kjeldahl flask. Add 25 ml concentrated sulphuric acid, approximately 0 .5 g copper sulphate and 5 mg selenium and 10 g anhydrous sodium sulphate or potassium sulphate. Heat gently over a small tlame untll frothmg ceases and the hqmd 1s practically colourless. Continue to heat for a further two hours. Avoid local overheating. If frothing is excessive, add about 0 .5 g paraffin wax. Dissolve the cooled digest in water, and make up to a total volume of about 250 ml. Taking precautions against loss of ammonia, add sufficient sodium hydroxide solution to neutralise the acid and 10 ml in excess; mix well and connect immediately to a distillation apparatus. Distil into 10 ml of boric acid Indicator solution, diluted with 20 ml water controlling the rate of distillation so that not less than 150 ml d1stlls m thirty mmutes. 'l '1trate the d1stlllate agamst the standard 0.2 N acid solution. Carry out a similar detennination using all the reagents omitting only the sample. Calculate the total nitrogen content of the sample. 1 ml 0.2 N acid 0.0028 gnitrogen. 3.4 Total nitrogen (organic, ammoniacal and nitrate) in the presence of nitrate Weigh to the nearest mg. about 2 g of the sample ( or such an amount as shall 441 THE SUBSIDIARY LAWS OF BELIZE [ Prin.ted by the Governm.ent Printer, ] No. I Power Lane, REVISED EDITION 2003 Belmopan, by the authority of the Government of Belize. 442 CAP. 211] Belize Agricultural Health Authority contain not more than 250 mg nitrogen), transfer to a 500 mi Kj eldahl flask, add 3 g Devarda alloy and wash down the inside wall of the flask with 50 ml water. Close the flask with a rubber stopper provided with funnel and a delivery tube connected with 2 "U"-tubes (with bulbs) in series, each containing 10 ml 10% sulphuric acid. Add 5 ml 50% sodium hydroxide solution through the tap funnel. Allow to stand for thirty minutes and then heat up just below boiling point for sixty minutes. Cool, add 20 ml 50% sulphuric acid through the tap funnel, such that the sides of the flask are washed down by the acid. Remove the rubber stopper, wash the contents of the "U"-tubes into the Kjeldahl flask, add 30 ml concentrated sulphuric acid and heat until all the water has boiled off. Heat gently over a small flame until the solution is clear and then heat for a further two hours. If frothing is excessive add 0.5 gparaffin wax. Cool, carefully dilute with water, cool and transfer quantitatively to 25 0 mi volumetric flask. Dilute to 250 ml, mixing well and transfer an aliquot of 100 ml to a 500 ml distillation flask. Add 200 mi water and 500/c sodium hydroxide solution, until the solution is neutral, cooling during the addition. Add an additional 10 ml 50% sodium hydroxide, quickly close the distillation flask and distil about 150 ml into 10 ml boric acid/indicator solution diluted with 20 ml water. Titrate the distillate against the standard 0.2 N acid solution. Carry out a similar determination using all the reagents omitting only the sample. Calculate the total nitrogen content of the sample. 1 ml 0.2 N acid- 0.0028 g nitrogen. 4. Determination of Phosphoric acid For the purposes of the Act, Part IV, "phosphoric acid" means P 2 0 5 ( molecular weight 142.04). Phosphoric acid shall be determined by the spectrophotometric, (vanadium phosphomolybdate) method. The spectrophotometric method compares the amount oflight transmitted by the solution to that by a solution of known phosphoric acid content. The determination is carried out differentially in order to increase the accuracy. Preferably an instrument with a monochromator giving a source oflight with a THE SUBSIDIARY LAWS OF BELIZE [ Printed by the Government Printer,] No. 1 Power Lane, Belmopan, by the authority of the Government of Belize. REVISED EDITION 2003 Belize Agricultural Health Authority [CAP.211 wavelength of 420 nm is required; alternatively a filter instrument can be used. 4.1 Spectrophotometric (Vanadium Phosphomolybdate) Method 4.11 Reagents Calcium oxide - finely ground. Hydrochloric acid, concentrated ( d-1.18). Nitric acid, concentrated(d-1.42). Potassium dihydrogen phosphate solution ( stock phosphate solution) Dissolve in water 1.917 g potassium dihydrogen phosphate previously dried at 105°C for 1 hour and dilute to 1 litre. Potassium dihydrogen phosphate solution ( standard phosphate solution) Dilute 50 ml stock solution to 250 ml with water. (1 ml- 0.2 mg phosphoric acid, P 2 0 5 ). Sodium hydroxide, N. Vanado-molybdate reagent - Dissolve separately 20 g ammonium molybdate and 1 g ammonium vanadate in water, mix, acidify with 140 ml concentrated nitric acid and dilute to 1 litre. 4.12 Total phosphoric acid in fertilizers 4.121 Dissolution of the sample 4.1211 In the absence of organic matter Weigh to the nearest mg. about 5 g of the sample into a 400 ml beaker, add 100 ml water and stir thoroughly. Boil the mixture, add slowly to the boiling solution 10 ml concentrated hydrochloric acid in a thin stream, and then 10 ml concentrated nitric acid; boil gently for ten minutes, cool, transfer to a 500 ml volumetric flask and dilute to the mark with water. Mix well and filter the solution through a dry filter paper into a dry flask, discarding the first 10 or 20 ml. Retain the rest of the filtrate. 443 THE SUBSIDIARY LAWS OF BELIZE [ Prin.ted by the Governm.ent Printer, ] No. I Power Lane, REVISED EDITION 2003 Belmopan, by the authority of the Government of Belize. 444 CAP. 211] Belize Agricultural Health Authority 4.1212 In the presence of organic matter Weigh to the nearest mg. about 5 g of the sample into a capsule or dish of about 5 cm in diameter; add I g calcium oxide and mix well with a stout platinum wire or thin glass rod. Calcine the mixture at a temperature not exceeding 500°C to destroy the organic matter. Allow the capsule or dish to cool and transfer the contents to a 400 ml beaker; add 100 ml water, stir thoroughly and heat to boiling point. Add slowly to the boiling solution 10 ml concentrated hydrochloric acid, and then 10 ml concentrated nitric acid, and boil gently. If the solution is clear, continue to boil gently for ten minutes, then cool, transfer to a 500 ml volumetric flask, and dilute to the mark. If the solution shows the presence of carbonaceous matter, filter the solution, wash the insoluble matter with a little water, and then transfer the filter paper containing the insoluble matter to the capsule or dish and calcine until all the carbon is destroyed. Allow to cool and transfer the contents to the filtrate; heat to boiling point and gently boil for ten minutes. Then cool, transfer to a 500 ml volumetric flask and dilute to the mark. Filter. 4.122 Procedure 4.1221 Standardisation ofinstrument From a burette, measure into a series ofl00 ml volumetric flasks 25.0, 26.0, 27 .0, 28.0, 29 .0, 30.0, and 31.0 ml of the standard phosphate solution (i.e. 5.0, 5.2, 5.4, 5.6, 5.8, 6.0 and 6.2 mg phosphoric acid). Add 25 ml of the vanado-molybdate reagent to each flask and dilute to 100 ml with water making sure that the temperature of the reagent and the dilution water is 20°C. Shake and allow to stand for ten minutes. Set the spectrophotometer to the correct wavelength, circa 420 nm, fill two 1 cm cells with the 5 .0 mg solution and check the extinction of the cell. If there is a small difference, select the cell with the smaller reading as the standard THE SUBSIDIARY LAWS OF BELIZE [ Printed by the Government Printer,] No. 1 Power Lane, Belmopan, by the authority of the Government of Belize. REVISED EDITION 2003 Belize Agricultural Health Authority [CAP.211 reference cell. Determine the apparent extinction at 20°C ( correct for cell differences) of the 5.2, 5.4, 5.6, 6.0 and 6.2 mg phosphoric acid solutions referred to the 5.0 mg phosphoric acid solution as standard. Plot a calibration graph of scale reading against known phosphoric acid content. 4.1222 Analysis of sample Successively dilute a portion of the solution prepared according to paragraph 4.1211 or paragraph 4.1212 so that the final volume of about 25 ml contains between 5.5 and 6.2 mg phosphoric acid, taking care that the dilution water is at a temperature of20°C. Transfer this final volume to a 100 ml volumetric flask, add 25 ml of the vanado-molybdate reagent ( at a temperature of 20°C), dilute to the mark, mix, and allow to stand for ten minutes. At the same time transfer 25 ml of the standard phosphate solution ( at 20°C) into a second 100 ml volumetric flask. Add 25 ml of the vanado-molybdate reagent ( at 20°C), dilute to the mark, mix, and allow to stand for ten minutes. Measure the difference in extinction at 20°C between the two solutions and estimate the phosphoric acid content of the volume of the unknown solution from the calibration graph. Calculate the phosphoric acid content of the sample from known dilution factors and the weight of the sample. NOTE: Prepare a fresh reference standard for each series of readings on the instrument. 445 THE SUBSIDIARY LAWS OF BELIZE [ Prin.ted by the Governm.ent Printer, ] No. I Power Lane, REVISED EDITION 2003 Belmopan, by the authority of the Government of Belize. 446 CAP. 211] Belize Agricultural Health Authority 5. Determination of Potash For the purposes of the Act, "potash" means potassium oxide (~ 0) Potash in all kinds of fertilizers may be determined by the flame photometric method. 5.1 Flame Photometric Method The determination of potash by this method depends on the measurement of the characteristic radiation emitted from a flame into which a solution of the sample is sprayed. The chosen radiation lie in the spectral range 766-770 nm. These radiations may be isolated by either a monochromator or the use of a suitable filter. 5.11 Reagents Ammonia solution, 30% v/v - Dilute 30 ml concentrated ammonia solution (d-0.88) with water to 100 ml. Ammonium oxalate solution - saturated aqueous solution. Hydrochloric acid, concentrated ( d-1.18). Potassium dihydrogen phosphate solution ( stock potash solution) Dissolve in water 5. 779 g potassium dihydrogen phosphate previously dried for one hour at 105°C and dilute to 1 litre. Potassium dihydrogen phosphate solution (standard potash solutions) Dilute 50 ml stock solution to 1 litre with water. This solution contains 100 ppm potash (~0). 5. 12 Potassium salts If the salts contain calcium, iron, aluminium or other interfering substances, the procedure described in paragraph 5 .13 should be used instead of the following procedure. THE SUBSIDIARY LAWS OF BELIZE [ Printed by the Government Printer,] No. 1 Power Lane, Belmopan, by the authority of the Government of Belize. REVISED EDITION 2003 Belize Agricultural Health Authority [CAP.211 Weigh to the nearest mg. about 2.5 g of the sample and transfer to a 400 ml beaker. Add 5 ml concentrated hydrochloric acid 50 ml water and bring the contents to the boiling point, breaking down with a stirring rod any crystals or lumps. Dilute the solution with water to about 100 ml and boil gently for a few minutes. Cool the solution to 20°C, transfer to a 250 ml volumetric flask, and dilute to the mark. Mix and filter through a dry filter paper. Successively dilute so that the final solution contains approximately 16 ppm potash and determine the potash in the filtrate by the method described in paragraph 5 .15. 5.13 Potash in mixed fertilizers containing little or no organic matter Weigh to the nearest mg. about 2.5 g of the sample and transfer to a 400 ml beaker. Add 50 ml of water and 5 ml concentrated hydrochloric acid and evaporate to dryness on a water bath. Add 125 ml water and 50 ml ammonium oxalate solution. Boil the contents for thirty minutes. If necessary, a small quantity of a potassium-free anti-foaming agent may be added. Cool the liquid, add a slight excess of ammonia solution and cool to 20°C. Transfer to a 250 ml vol1m1etric flask, and dilute to the mark. Mix the solution and filter through a dry filter paper. Successively dilute so that the final solution contains approximately 16 ppm potash in the filtrate by the method described in paragraph 5 .15. 5.14 Potash in mixed fertilizers containing organic matter Weigh to the nearest centigram about 10 g of the sample and gently incinerate at a temperature not exceeding 5 00°C in order to destroy the organic matter. Grind the residue to eliminate lumps, add 50 ml of water, 10 ml of concentrated hydrochloric acid, and evaporate to dryness on a water bath. Boil the residue for thirty minutes, with 125 ml water and 50 m ammonium oxalate solution. Cool the solution, add a slight excess of an1monia solution, cool to 20°C, transfer to a 500 ml volumetric flask and dilute to the mark. Mix the solution and filter through a dry filter paper. Successively dilute so that the final solution contains approximately 16 ppm potash and determine the potash in the filtrate by the method described in paragraph 5 .15. 447 THE SUBSIDIARY LAWS OF BELIZE [ Prin.ted by the Governm.ent Printer, ] No. I Power Lane, REVISED EDITION 2003 Belmopan, by the authority of the Government of Belize. 448 CAP. 211] Belize Agricultural Health Authority 5.15 Determination of Potash by Flame Photometry 5.151 Calibration ofinstrument From the standard potash solution, prepare a set of aeeurate dilutions containing 10, 12, 14, 16, 18 and 20 ppm potash. Set the sensitivity of the flame photometer so that 100 scale divisions (full scale deflection) is equivalent to 20 ppm. potash solution. Spray the 10, 12, 14, 16, and 18 ppm potash solutions three times. Take the median reading ( not the mean), and construct a calibration graph. After spraying each different strength solution, again spray the 20 ppm solution to ensure that the sensitivity of the flame photometer has not changed. 5.152 Analysis of sample Resetthe instrument at 100 scale divisions (full scale deflection) with 20 ppm potash solution. Spray the diluted fertilizer solution prepared in accordance with paragraph 5.12, 5.13 or 5.14 and read from the graph the approximate potash content of the solution. Prepare two further dilutions of the standard potash solution to contain respectively 1 ppm more and 1 ppm less potash than the estimated potash content of the diluted solution of the sample. Successively spray the low standard solution, the diluted solution of the sample, and the high standard solution. Repeat this operation twice more. Take the median result of each set of three readings and calculate the potash content of the sample solution and hence of the fertilizer from the proportionality of the radiation given by the sample solution and that given by the two standard solutions containing respectively 1 ppm more and 1 ppm less potash than the predicted potash content. NOTE: It is essential that the flame photometer should be set up in a vibration-free position and in a dust-free atmosphere. Dilute standard solutions should be freshly prepared. THE SUBSIDIARY LAWS OF BELIZE [ Printed by the Government Printer,] No. 1 Power Lane, Belmopan, by the authority of the Government of Belize. REVISED EDITION 2003 Belize Agricultural Health Authority [CAP.211 6. Determination of neutralising value in liming materials 6.1 Reagents Hydrochloric acid, 0.5 N. Phenolphthalein indicator solution -Dissolve 0.25 g phenolphthalein in 150 ml industrial methylated spirit and dilute with water to 250 ml. Sodium hydroxide,0.5 N - carbonate free. 6.2 Prepare the sample as described in paragraph 1.11 6.3 Procedure Weigh to the nearest mg. about 500 mg of the sample prepared according to paragraph 6.2 and transfer to a 300 ml flask. Add 50 ml 0.5 N hydrochloric acid, cover the flask with a glass and boil the contents gently for five minutes. Cool the mixture, add two or three drops of the phenophthalein indicator solution and titrate with 0.5 N sodium hydroxide solution. Calculate by difference the volume of0.5 N hydrochloric acid required to neutralise the sample. Express the result as percentage by weight of calcium oxide (CaO). 1 ml 0.5 N hydrochloric acid 0.01402 g calcium oxide (CaO). 7. Determination of magnesium in lime and ground limestone 7.1 Reagents Ammonia solution, 25% v/v - Dilute 30 ml concentrated aimnonia solution ( d-0.91) with water to 100 ml. Ammonium chloride solution -Dissolve 330 g ammonium chloride in water and dilute to 1 litre. Ammonium persulphate solution -Dissolve 10 g ammonium persulphate in water and dilute to 100 ml. Store in a cool dark place for not more than one week. 449 THE SUBSIDIARY LAWS OF BELIZE [ Prin.ted by the Governm.ent Printer, ] No. I Power Lane, REVISED EDITION 2003 Belmopan, by the authority of the Government of Belize. 4so CAP. 211] Belize Agricultural Health Authority Buffer solution - Dissolve 6.75 g ammonium chloride, 62 mg magnesium sulphate (MgS0 4 .7H 2 0) 93 mg disodium ethylenediamine-tetra-acetate dihydrate and 57 ml ammonia solution ( d-0.88) in water and dilute to 100 ml. Calcium standard solution- Dissolve 2.5 g calcium carbonate in 125 ml 0.5 N hydrochloric acid and dilute to 1 litre. EDTAsolution, 0.025 M- Dissolve 10 g disodium ethylenediaminetetra-acetate dihydrate in 800 ml water containing 55 ml N sodium hydroxide solution. Dilute 20 ml standard calcium solution with 30 ml water. Add 1 ml buffer solution and 200 mg Mordant Black 11; titrate with the EDTA solution to a blue end point and adjust the strength of this solution so that 1 ml is equivalent to 2.5 mg calcium carbonate (CaC0). Hydrochloric acid, 0.5 N. Hydrogen peroxide, 6% v/v (20 volume). Mordant Black 11 indicator ( colour index No. 14645) - Mix 200 mg Mordant Black 11 and 50 g sodium chloride uniformly and together grind to pass through a sieve having apertures of about 0.3 min square. Murexide indicator- Mix 200 mg Murexide and 100 g sodium chloride uniformly together and grind to pass through a sieve having apertures of about 0.3 mm square. Protect this mixture from light. Sodium hydroxide, N. 7 .2 Procedure Weigh to the nearest mg. about 1 g finely ground sample and add 50 ml 0. 5 N hydrochloric acid. Transfer to a conical flask, cover with a glass and boil for 3 minutes.Add2 ml hydrogen peroxide solution, reboil, cool, add 1 ml ammonium chloride solution, a slight excess of 25 % ammonia solution and 1 ml ammonium persulphate solution. Remove the excess ammonia by boiling and filter the precipitate, if any, on a small paper and wash with two portions each of 10 ml THE SUBSIDIARY LAWS OF BELIZE [ Printed by the Government Printer,] No. 1 Power Lane, Belmopan, by the authority of the Government of Belize. REVISED EDITION 2003 Belize Agricultural Health Authority [CAP.211 hot water. Wash the precipitate off the paper with not more than 50 ml water, and boil with 50 ml 0.5 N hydrochloric acid. Cool the solution, add 1 ml ammonium chloride solution, a slight excess of dilute ammonia and 1 ml ammonium persulphate solution and remove the excess of ammonia by boiling. Filter and wash with hot water. Add the filtrate and washings to the filtrate and washing from the first precipitation, cool and dilute the whole to 200 ml. Ifno precipitate forms on the addition of the ammonia and persulphate solutions, remove the excess of ammonia by boiling, add 6 ml ammonium chloride solution, cool and dilute to 200 ml. If the amount of the precipitate is small, omitthe second precipitation but add 6 ml ammonium chloride solution to the filtrate and washings before cooling and diluting to 200 ml. Dilute 20 ml of the solution to 50 ml and add 3 ml 25% ammonia solution. Then add 200 mg Mordant Black 11 indicator and titrate with EDTA solution to a blue end point. Dilute a further 20 ml of the solution to 50 ml and add 7 ml N sodium hydroxide. Then add 200 mg Murexide indicator and titrate with EDTAsolution to a violet endpoint. Calculate the magnesium content from the difference between the two titrations. 1 ml EDTAsolution 0.608 mg magnesium. 8. Determination of Boron For levels above 1,000 ppm, boron is detennined by titration as boric acid and for levels up to 1,000 ppm by the carmine spectrophotometric method. 451 THE SUBSIDIARY LAWS OF BELIZE [ Prin.ted by the Governm.ent Printer, ] No. I Power Lane, REVISED EDITION 2003 Belmopan, by the authority of the Government of Belize. 4s2 CAP. 211] Belize Agricultural Health Authority 8.1 Titrimetric Method 8.11 Reagents Calcium oxide. Hydrochloric acid, 50% v/v - Dilute 50 ml concentrated hydrochloric acid ( d = 1. 18) with water to 100 ml. Hydrochloric acid, 0.5 N. Lead nitrate solution -Dissolve 10 g lead nitrate in water and dilute to 100 ml. Mannitol. Methylated indicator solution - Dissoive 0.025 g methyl red in 5 ml 90% industrial methylated spirit with the aid ofO .5 ml 0.1 N sodium hydroxide. Dilute to 250 ml with 50% industrial methylated spirit. Sodium carbonate. Sodium hydroxide, 0.5 N. Sodium hydroxide, 0.05 N - Prepare from a 50% solution which has been allowed to settle. Use boiled and cooled water for dilution. Store in a polythene bottle protected from the atmosphere by a guard tube and fitted with a syphon forwithdrawingthe solution. 8.12 Dissolution of the sample 8.121 In the absence of organic matter Weigh to the nearest mg. about 2 g of the sample, ifit contains 0.5% or less of boron, and l g if it contains from 0.5 - 1.0% of boron. Transfer to a 400 ml beaker. Add 100 ml water and some phenolphthalein indicator. Add sodium carbonate to make the solution slightly alkaline and boil gently. Keep the boiling solution just alkaline by further additions of sodium carbonate until all the ammonia which may be present has been evolved. Cool the solution, add 12 ml 50% hydrochloric acid. THE SUBSIDIARY LAWS OF BELIZE [ Printed by the Government Printer,] No. 1 Power Lane, Belmopan, by the authority of the Government of Belize. REVISED EDITION 2003 Belize Agricultural Health Authority [CAP.211 8.122 In the presence of organic matter Weigh to the nearest mg. about 2 g of the sample, if it contains 0.5% or less of boron, and 1 g if it contains from 0 .5 - 1. 0% of boron. Place in a silica dish, add 0.2 g calcium oxide each 1 g of the sample, for moisten with water, mix thoroughly, evaporate the mixture to dryness and ignite gently in a muffle furnace at 450°C. Allow the washing to proceed for about three hours. Cool. Moisten with 10 ml 50% hydrochloric acid, warm on a water bath for fifteen minutes, covering the dish with a watch glass. Transfer to a 400 ml beaker, add a few drops of phenolphthalein indicator and dilute to about 120 ml with water. 8.13 Procedure To the solution prepared in accordance with paragraph 8.121 or 8.122, add 20 ml lead nitrate solution for each 12%P 2 0 5 in the sample if2 g of the sample have been used and 10 mlleadsolution for each 12% P 2 0 5 in the sample ifl g of the sample has been used. Heat just to boiling, remove from source of heat and make just allrnline by adding solid sodium carbonate. Stand on a water bath for five minutes. Cool, transfer to a 200 ml volumetric flask and dilute to the mark with water. Mix and filter through a 24 cm Whatman No. 42 ( or equivalent) filter paper, rejecting the first 10-20 ml of the filtrate. Transfer 100 ml of the filtrate to a 250 ml beaker. Add a few drops of methyl red indicator and acidify the solution with 0.5 N hydrochloric acid. Heat almost to boiling and stir vigorously to remove carbon dioxide, adding a little more 0.5 N hydrochloric acid if the colour changes to orange orto yellow. Neutralise to methyl red with 0.5 N sodium hydroxide and make just acid with 0.5 N hydrochloric acid. Cover with a watch glass and boil gently for five minutes to expel any remaining carbon dioxide. Cool rapidly. Place the electrodes of potentiometric titration apparatus in the beaker and adjust the pH to 6.3 by adding 0.05 N sodium hydroxide solution. Add 10 g mannitol and titrate with 0.005 N sodium hydroxide solution to final pH of 6.3. Add a further quantity of mannitol and continue the titration to a pH of 6.3. Further additions of mannitol should not alter the pH. Let x ml of0.05 N sodium 453 THE SUBSIDIARY LAWS OF BELIZE [ Prin.ted by the Governm.ent Printer, ] No. I Power Lane, REVISED EDITION 2003 Belmopan, by the authority of the Government of Belize. 454 CAP. 211] Belize Agricultural Health Authority hydroxide be used for the titration after the addition of the mannitol. Allow a standard value ofO. 1 ml 0.05 N sodium hydroxide solution as "blank" value. Calculate Boron: % Boron in sample 0. 1082 ( X - OJ) Weight of sample taken 8.2 Spectrophotometric (Carmine) Method 9.2 Reagents Boric acid (stock boron solution- Dissolve 1.905 g boric acid in water and dilute to 1,000 ml at 20°C. 1 ml= 0.333 mg boron. Boric acid ( standard boron solution) - Dilute 10 ml stock solution with water to 100 ml at20°C. Transfer 5, 10, 15, 20 and25 ml of this dilute solution to 100 ml volumetric flasks and dilute to the marks with water. These standards will contain 5, 10, 15, 20, 25 ug of boron per 3 ml. Calcium oxide. Establish the calibration graph as follows: Measure amounts of standard cobalt solution corresponding to 0, 3, 6, 9, 12, 15 ug of cobalt into a series of 100 ml beakers and proceed as described above commencing at "Add 15 ml sodium citrate solution." Measure the extinctions to the solutions, and construct a graph relating the extinctions to the number of micrograms of cobalt. THE SUBSIDIARY LAWS OF BELIZE [ Printed by the Government Printer,] No. 1 Power Lane, Belmopan, by the authority of the Government of Belize. REVISED EDITION 2003 Belize Agricultural Health Authority [CAP.211 10. Determination of Copper Copper may be determined by the diethyldithiocarbam ate spectrophotometric method or, alternatively, by the atomic absorption spectrophotometric method. 10.1 Diethyldithiocarbamate spectrophotometric method 10.11 Reagents Ammonia solution, approximately 6 N - This may be prepared by passing gaseous ammonia into distilled water, or by purifying ammonia solution as described for EDTA-citrate solution below. Carbon tetrachloride, redistilled. Copper sulphate, stock solution - Dissolve 0.393 g copper sulphate, CuS0. 4 .5H 2 0, in 100 ml 2 N sulphuric acid and dilute to 1 litre at 20°C with distilled water. Copper sulphate standard solution - Dilute 5 ml stock solution to 250 ml with 2 N sulphuric acid at 20°C immediately before use. 1 ml= 2 ug copper. EDTA-citrate solution - Dissolve 20 g ammonium citrate and 5 of the disodium salt of ethylenediamine-tetra-acetic acid (EDTA) in distilled water and dilute to 100 ml. To purify, add 0.1 ml sodium diethyldithiocarbamate solution and extract with carbon tetrachloride. Add a further quantity of sodium diethy 1 dithiocarbamate solution to ensure that it is in excess. Sodium diethyldithiocarbariiate solution - Dissolve 1 g sodium diethy 1 dithiocarbamate in distilled water and dilute to 100 ml; Filter the solution if it is not clear. Store the solution in the dark in a refrigerator and discard after seven days. 455 THE SUBSIDIARY LAWS OF BELIZE [ Prin.ted by the Governm.ent Printer, ] No. I Power Lane, REVISED EDITION 2003 Belmopan, by the authority of the Government of Belize. 456 CAP. 211] Belize Agricultural Health Authority Sodium hydroxide, 0. 1 N. Sulphuric acid, 2 N. Thymol blue indicator solution-Dissolve 0.1 gthymol blue in2.15 ml 0.1 N sodium hydroxide and dilute to 100 ml with water. 10.12 Dissolution of the sample Prepare a solution of the sample as described in paragraph 9 .2. 10.13 Procedure Transfer to a separating funnel a suitable aliquot ( containing not more than 50 ug of copper) of the solution prepared in accordance with paragraph 9 .2. Add 10 ml EDTA-citrate solution, 2 drops of thymol blue indicator solution and ammonia solution until the mixture is coloured green or bluish-green. Cool the mixture, add 1 ml sodium diethyldithiocarbamate solution and, from a burette, 15 ml carbon tetrachloride. Stopper the funnel, shake vigorously for two minutes and allow the layers to separate. Place a piece of cotton-wool in the stem of the funnel and run off the carbon tetrachloride layer into a dry spectrophotometer cell. Avoid undue exposure of the solution to light. Simultaneously with the test determination, carry out a blank determination on all the reagents used. Measure immediately the extinctions of the test and blank solutions at a wavelength of 436 nm, using carbon tetrachloride in the comparison cell. Read from a previously prepared calibration graph the number of micrograms of copper corresponding to the observed extinctions of the test and blank solutions, and so obtain by difference the net measure of copper in the sample. Establish the calibration graph as follows: THE SUBSIDIARY LAWS OF BELIZE [ Printed by the Government Printer,] No. 1 Power Lane, Belmopan, by the authority of the Government of Belize. REVISED EDITION 2003 Belize Agricultural Health Authority [CAP.211 To a series of separating funnels transfer 10 ml EDTA-citrate solution and the following amounts of standard copper solution and 2 N sulphuric acid: Copper solution ..... 0 1 2.5 5 10 15 20 25 ml 2 N sulphuric acid 25 24 22.5 20 15 10 5 0ml Proceed as for the test solution, as described above, commencing at "2 drops ofthymol blue ... ". Measure the extinctions of the solutions and construct a graph relating the extinctions to the number of micrograms of copper. 10.2 Atomic absorption spectrophotometric method 10.21 Apparatus Atomic absorption spectrophotometer. Copper hollow-cathode lamp. 10.22 Reagents Copper sulphate standard solution - Dissolve 0.393 g copper sulphate CuS0 4 .5H 2 0, in 0.5 N hydrochloric acid and dilute to 100 ml with 0.5 N hydrochloric acid, 1 ml= 1 mg copper. Dilute this solution as required. Hydrochloric acid, 0.5 N. 10.23 Procedure Set up the instrument using the line at 3 24. 7 nm. Prepare from the standard copper solution a series of solutions in 0.5 N hydrochloric acid, containing between 0 and 10 ppm copper. Dilute a suitable aliquot of the sample solution, prepared in accordance with paragraph 9 .2, with 0.5 N hydrochloric acid to produce a standard volume of solution containing between 0 and 10 ppm copper. Prepare a blank solution from which only the sample has been omitted. Spray distilled water into the flame and zero the instrument. Spray successively, in 457 THE SUBSIDIARY LAWS OF BELIZE [ Prin.ted by the Governm.ent Printer, ] No. I Power Lane, REVISED EDITION 2003 Belmopan, by the authority of the Government of Belize. 458 CAP. 211] Belize Agricultural Health Authority triplicate, the standard solutions, sample and blank, washing the instrument through with distilled water between each spraying. Record the absorbance reading or the peak high on the recorder if a recording instrument is used. Plot the mean reading obtained for each standard solution against its copper content. Determine the copper content of the sample and blank solutions from the graph and from the difference between them calculate the copper content of the sample. If a large number of samples is being examined one or more standard solutions must be resprayed at intervals during the course of the analyses. 11. Determination oflron For levels up to 1 % iron is determined by the o-phenanthroline spectrophotometric method and for levels above 1 % by the titrimetric method with potassium di chromate. 11. 1 o-Phenanthroline Method 11.11 Reagents Ammonium ferric sulphate solution (stock iron solution)- Dissolve 0.863 g ammonium ferric sulphate, Fe 2 (S0 4 \. (NH 4 \SO 4 .24H 2 0, in water containing 2 ml perchloric acid, and dilute to 100 ml at 20°C. Ammonium ferric sulphate solution (standard iron solution) - Dilute 10 ml stock solution to l 00 ml with water at 20°C immediately before use. 1 ml = l00mgiron. Bromophenol blue indicator solution - Dissolve 0.4 g bromophenol blue in 95% ethanol and dilute to 100 ml. Hydrochloric acid, 50% v/v - Dilute 50 ml concentrated hydrochloric acid ( d = 1.18) with water to 100 ml. THE SUBSIDIARY LAWS OF BELIZE [ Printed by the Government Printer,] No. 1 Power Lane, Belmopan, by the authority of the Government of Belize. REVISED EDITION 2003 Belize Agricultural Health Authority [CAP.211 Hydrochloric acid, 20% v/v - Dilute 20 ml concentrated hydrochloric acid ( d =. 1. 18) with water to 100 ml. Nitric acid, 3096v/v-Dilute 30mlconcentratednitric acid(d 1.42)with water to 100ml. o-Phenanthroline solution - Dissolve 0.25 g o-phenanthroline in 25% ethanol and dilute to 100 ml. Quinol solution - Dissolve 1 g quinol in water and dilute to 100 ml. Sodium citrate solution - Dissolve 25 sodium citrate in water and dilute to 100 ml 11.12 Dissolution of the sample Prepare a solution of the sample as described in paragraph 9 .2. 11.13 Procedure Transfer a suitable aliquot of the solution, prepared in accordance with paragraph 9 .2, to a small flask, add a few drops of the bromophenol blue indicator solution, and titrate with sodium citrate solution until the colour changes from yellow to blue. Transfer another aliquot to a 25 ml volumetric flask, add 1 ml solution, 3 ml o-phenanthroline solution and an amount of sodium citrate solution equal to the above titration, and then dilute with water to 25 ml. Allow the solution to stand for 1 hour. Carry out a blank detennination on all the reagents used. Measure the extinctions of the test and blank solutions at a wavelength of 510 nm using 4 cm or 1 cm cells according to the depth of colour with water in the comparison cell. Read the number of micrograms of iron equivalent to the observed extinctions of the test and blank solutions from a previously prepared 459 THE SUBSIDIARY LAWS OF BELIZE [ Prin.ted by the Governm.ent Printer, ] No. I Power Lane, REVISED EDITION 2003 Belmopan, by the authority of the Government of Belize. 460 CAP. 211] Belize Agricultural Health Authority calibration graph, and so obtain the net measure of iron in the sample. Establish the calibration graph as follows: Measure amounts of standard iron solution corresponding to 0, 200, 300, 400, 500, 600 ug of iron into a series of 100 ml volumetric flasks. To each add 50 ml 20% v/v hydrochloric acid, and dilute to 100 ml with water. Using 5 ml aliquots, proceed as for the test solution, as described above commencing at "Transfer a suitable aliquot of the solution ...... ". Measure the extinctions of the solutions and construct a graph relating the extinctions to the number of micrograms of iron. 11.2 Titrimetric Method 11.21 Reagents Hydrochloric acid, concentrated ( d = 1.18). Hydrochloric acid, 30% v /v - Dilute 30 ml concentrated hydrochloric acid ( d = 1.18) with water to 100 ml. Mercuric chloride solution -Dissolve 5 g mercuric chloride in water and dilute to 100 ml. Orthophosphoric acid, concentrated ( d = 1. 7 5). Potassium dichromate, 0.1 N. Sodium diphenylamine-4-sulphonate indicator solution -Dissolve 0.2 g sodium diphenylamine-4-sulphonate in water and dilute to 100 ml. Stannous chloride solution- Dissolve 15 g stannous chloride dihydrate in 30% v /v hydrochloric acid and dilute to 100 ml with 3 0% v /v hydrochloric acid. THE SUBSIDIARY LAWS OF BELIZE [ Printed by the Government Printer,] No. 1 Power Lane, Belmopan, by the authority of the Government of Belize. REVISED EDITION 2003 Belize Agricultural Health Authority [CAP.211 This solution should be prepared immediately before use. Stannous chloride, dilute - Dilute 5 ml stannous chloride solution with 30% v/v hydrochloric acid to 50 ml. Sulphuric acid, 16% v/v- To 50 ml water cautiously add 16 ml concentrated sulphuric acid ( d = 1.84 ). Cool and dilute to 100 ml. 11.22 Dissolution of the sample Prepare a solution of the sample as described in paragraph 9 .2. 11.23 Procedure Transfer a suitable aliquot of the solution, prepared in accordance with paragraph 9 .2, to a 500 ml flask and dilute or concentrate the solution to about 20 ml. Add concentrated hydrochloric acid so that the total amount of acid present is equivalent to about 5 ml concentrated hydrochloric acid. Heat the solution to 70 - 90°C add the - stannous chloride solution drop wise until the yellow colour has almost disappeared. Continue the addition using diluted stannous chloride solution until the solution becomes colourless or slightly green and add one or two drops more. Cool the solution rapidly to room temperature, and add 10 ml mercuric chloride solution. A small, white 'silky' looking precipitate should fom1. (If no precipitate forms, insufficient stannous chloride has been added; on the other hand if the precipitate is grey or black to much stannous chloride has been added. In either case the solution must be discarded). Add 200 ml water, 10 ml 16% v/v sulphuric acid, 5 ml orthophosphoric acid and 6-8 drops of indicator. Titrate with 0 .1 N potassium dichromate until the indicator changes from green to violet-blue. Calculate the amount of iron in the sample using the factor 1 ml 0.1 N potassium di chromate = 0.00559 g iron. 461 THE SUBSIDIARY LAWS OF BELIZE [ Prin.ted by the Governm.ent Printer, ] No. I Power Lane, REVISED EDITION 2003 Belmopan, by the authority of the Government of Belize. 462 CAP. 211] Belize Agricultural Health Authority 12. Determination of Magnesium Magnesium may be determined by the pyrophosphate method or, alternatively, by the atomic absorption spectrophotometric method. 12.1 Pyrophosphate Method 12.11 Reagents Ammonia solution, ( d = 0. 9 1). Ammonia solution, 5 % v/v - Dilute 5 ml concentrated ammonia solution ( d = 0.91) with water to 100 ml. Ammonium phosphate solution - Dissolve 20 g diammonium hydrogen phosphate, (NH 4 \ HP0 4 , in water and dilute to 100 ml. Ammonium oxalate solution - saturated aqueous solution. Calcium wash solution - Dissolve 1 g oxalic acid, (COOH\.2H 2 0 and 2 g ammonium oxalate, in water and dilute to 1,000 ml. Citric acid, monohydrate. Hydrochloric acid, concentrated(d= 1.18). Hydrochloric acid, 20% v/v - Dilute 20 ml concentrated hydrochloric acid ( d 1.18) with water to 100 ml. Methyl red indicator solution - Dissolve 0.025 g methyl red in 5 ml 90% industrial methylated spirit with the aid of0.5 ml 0.1 N sodium hydroxide. Dilute to 250 ml with 50% industrial methylated spirit. THE SUBSIDIARY LAWS OF BELIZE [ Printed by the Government Printer,] No. 1 Power Lane, Belmopan, by the authority of the Government of Belize. REVISED EDITION 2003 Belize Agricultural Health Authority [CAP.211 Oxalic acid solution - Dissolve 10 g oxalic acid, (COOH\.2H 2 0, in water and dilute to 100 ml. 12.12 Dissolution of the sample Prepare a soltition of the sample as described in paragraph 9 .2. 12.13 Procedure Transfer a suitable aliquot ( containing approximately 50 mg magnesium) of the solution, prepared in accordance with paragraph 9 .2, to a 500 ml beaker, and add 5% v/v ammonia solution until a slight precipitate is formed. Add citric acid, in small portions, until the precipitate just dissolves, and then 1 gin excess. Heat the solution to 40°C, add 0.2 ml ( 4 drops) of methyl red indicator solution, Neutralise with 5% v/v ammonia solution, and I ml in excess. Add oxalic acid solution until the mixture is just acid, and then 10 ml in excess. Boil the solution for one to two minutes, add 50 ml saturated ammonium oxalate solution, dilute if necessary, to 200 ml with distilled water, boil for a further minute and heat on a water bath for at least an hour. Filter through a Whatman No. 40 ( or equivalent) filter paper; wash the residue thoroughly with calcium wash solution. Combine the filtrate and washings, measure the volume, transfer to a beaker, and add, while stirring with a glass rod ( avoid touching the sides of the beaker with the rod), 20 ml of ammonium phosphate solution. While stirring continuously throughout, neutralise the solution with ammonia solution, added drop by drop from a burette, and add 20 ml in excess, together with a further 10 ml of ammonia solution for each 100 ml of solution in the beaker, Set the beaker aside for at least four hours or, preferably, overnight. Filter through a No. 4 sintered-silica crucible, and wash the residue with cold 5% v/v ammonia solution, ensuring that any precipitate adhering to the sides of the beaker and the glass rod is transferred to the crucible. Dry the crucible and residue, transfer to a cool muffle furnace, slowly raise the temperature to 950°C, and heat at this temperature for a half to one hour. Allow the crucible to cool in a desiccator, and weigh. Calculate the weight of the precipitate to its equivalent of magnesium 463 THE SUBSIDIARY LAWS OF BELIZE [ Prin.ted by the Governm.ent Printer, ] No. I Power Lane, REVISED EDITION 2003 Belmopan, by the authority of the Government of Belize. 464 CAP. 211] Belize Agricultural Health Authority by multiplying its weight by 0.2184. 12.2 Atomic absorption spectrophotometric method 12.21 Apparatus Atomic absorption spectrophotometer. Magnesium hollow-cathode lamp. 12.22 Reagents Hydrochloric acid, 0.5 N. Magnesium sulphate standard solution -Dissolve 1.013 g magnesium sulphate, MgSO 4 .7H 2 0, in 0.5 N hydrochloric acid and dilute to 100 ml with 0.5 N hydrochloric acid, 1 ml= 1 mg magnesium. Dilute this solution as required. Strontium chloride solution - Dissolve 15 g strontium chloride, SrC l 6.6H 2 0, in 0.5 N hydrochloric acid and dilute to 100 ml with 0.5 N hydrochloric acid. 12.23 Procedure Set up the instrument using the line at 285 .2 nm. Prepare from the standard magnesium solution a series. of solutions, in 0.5 N hydrochloric acid, containing between 0 and 3 ppm- magnesium (see Note). Dilute a suitable aliquot of the sample solution, prepared in acordance with paragraph 9.2 with 0.5 N hydrochloric acid to produce a standard volume of solution containing 0 and 3 ppm magnesium (see Note). Prepare a blank solution from which only the sample has been omitted (see Note). Spray distilled water into the flame and zero the instrument. Spray successively, in triplicate, the standard solution, sample and blank, washing the instrument with distilled water between each spraying. Record the THE SUBSIDIARY LAWS OF BELIZE [ Printed by the Government Printer,] No. 1 Power Lane, Belmopan, by the authority of the Government of Belize. REVISED EDITION 2003 Belize Agricultural Health Authority [CAP.211 absorbance reading, or the peak height on the recorder if a recording instrument is used. Plot the mean reading obtained for each standard solution against its magnesium content. Determine the magnesium content of the sample and blank solutions from the graph and from the difference between them calculate the magnesium content of the sample. If a large number of samples is being examined one or more standard solutions must be resprayed at intervals during the course of the analyses. NOTE: If the sample contains phosphate add strontium chloride solution at the rate of 5 ml for each 50 ml of diluted sample solution, before adjusting to standard volume. 13. Determination of Manganese 13.1 Reagents Orthophosphoric acid, concentrated ( d = 1. 7 5). Potassium periodate. Potassium permanganate (stock manganese solution) - Dissolve 0.288 g potassium permanganate in 100 to 200 ml water, add 5 ml 25% v/v sulphuric acid and dilute with water to 1 litre at 20°C. Potassium permanganate (standard manganese solution-Dilute 10 ml stock solution to 100 ml with water at 20°C inunediately before use. 1. ml= 10 ug manganese. Sulphuric acid, concentrated ( d = 1.84 ). Sulphuric acid, 25% v/v -To 50 ml water cautiously add 25 centrated sulphuric acid ( d = 1.84). Cool and dilute to 100 ml. 465 THE SUBSIDIARY LAWS OF BELIZE [ Prin.ted by the Governm.ent Printer, ] No. I Power Lane, REVISED EDITION 2003 Belmopan, by the authority of the Government of Belize. 466 CAP. 211] Belize Agricultural Health Authority 13.2 Dissolution of the sample Prepare a solution of the sample as described in paragraph 9 .2. 13.3 Procedure Transfer to a small beaker a suitable aliquot ( containing not more than 70 ug of manganese) of the solution prepared in accordance with paragraph 9 .2. Evaporate just to dryness at a low heat on a hot-plate, cool, add 10 ml water, 1.5 ml orthophosphoric acid and 1.5 ml concentrated sulphuric acid. Warm until the residue is dissolved and evaporate on the hot-plate at a low heat until the solution just fumes. Cool, add 3 ml water, warm again and transfer the solution to a glass-stoppered tube calibrated 10 ml. Wash the beaker with two further 3 ml quantities of water, adding these to the contents of the tube. (If there is a precipitate, allow the solution to stand and withdraw an aliquot of the clear supernatent liquid). Add 0 .5 g potassium periodate, adjustthe vollm1e of the solution to just above the 10 ml mark with water and heat the loosely stoppered tube in a boiling water bath for thirty minutes. Cool, and adjust the volume to the mark with water. Carry out a blank determination on all the reagents used. Measure the extinction of the test and blank solutions at a wavelength of 526 nm, using 1 cm cells with water, in the comparison cell. Read from a previously prepared calibration graph the number of micrograms of manganese corresponding to the observed extinctions of the test and blank solutions, and so obtain by difference the net measure of manganese in the sample. Establish the calibration graph as follows: Measure the amounts of the standard manganese solution corresponding to 0, 10, 20, 30, 40, 50, 60, 70 ug manganese into a series of glass-stoppered tubes calibrated at 10 ml. To each add 1.5 ml orthophosphoric acid and 1.5 ml concentrated sulphuric acid, and proceed as described above for the test solution, commencing at "Add 0.5 g potassium periodate ...... ". Measure the THE SUBSIDIARY LAWS OF BELIZE [ Printed by the Government Printer,] No. 1 Power Lane, Belmopan, by the authority of the Government of Belize. REVISED EDITION 2003 Belize Agricultural Health Authority [CAP.211 extinctions of the solution, and construct a graph relating the extinctions to the number of micrograms of manganese. 14. Determination of Molybdenum 14.1 Reagents Ammonium molybdate (stock molybdenum solution) - Dissolve 1.840 g ammoniummolybdate, (NH 4 \ M0 7 0 24 4H 2 0 in water and dilute 1,000 ml at 20°c. Ammonium molybdate standard molbydenum solution) - Dilute 1 ml stock solution to 1,000 ml with water at 20°C immediately before use. 1 ml - 1 ug molybdenum. Annnonium ferrous sulphate solution - Dissolve 4 g ammonium ferrous sulphate in water and dilute to 100 ml. Hydrochloric acid, N. Hydrochloric acid, 2 N. Potassium thiocynate solution - Dissolve 40 g potassium thiocyanate in water and dilute to 100 ml. Sodium sulphate, anhydrous. Solvent mixture - Mix equal volumes of carbon tetrachloride and 3-methylbutan-1-01. Stannous chloride solution - Suspend 40 g stannous chloride dihydrate in 20 ml 6.5 N hydrochloric acid, add water to dissolve and dilute to 100 ml. Filter if turbid. 467 THE SUBSIDIARY LAWS OF BELIZE [ Prin.ted by the Governm.ent Printer, ] No. I Power Lane, REVISED EDITION 2003 Belmopan, by the authority of the Government of Belize. 468 CAP. 211] Belize Agricultural Health Authority 14.2 Dissolution of the sampie Prepare a solution of the sample as described in paragraph 9.2 14.3 Procedure Transfer a suitable aliquot of the solution prepared in accordance with paragraph 9 .2 to a 125 ml separating funnel, add I ml ammonium ferrous sulphate solution and sufficient N hydrochloric acid to bring the volume to 50 ml ( see note), then add 1 ml potassium thiocynate solution and mix. Add 1 ml stannous chloride solution, and mix again. Add exactly 7 ml solvent mixture, shake vigorously for 2 minutes and allow to separate for fifteen minutes. Filter the lower layer through a 7 cm paper into a small stoppered tube. If the lower layer is not bright or if filtration is difficult, filter through a small suitable column packed with anhydrous sodium sulphate, solid stannous chloride and plugged with cotton wool. Carry out a blank determination on all the reagents used. Measure the extinction of the test and blank solutions at a wavelength of 470 nm, using 1 cm cells with water in the comparison cell. Read the number of micrograms of molybdenum equivalent to the observed extinctions of the test and blank solutions from a previously prepared calibration graph, and so obtain the net measure of molybdenum in the sample. Establish the calibration graph as follows: Measure amounts of the standard molybdenum solution corresponding to 0, 5, 10, 15, 20, 25 ugmolybdenuminto a series of 125 ml separating funnels. Add to each funnel 1 ml ammonium ferrous sulphate and 25 ml 2 N hydrochloric acid, dilute to 50 ml and proceed as for the test solution, as described above THE SUBSIDIARY LAWS OF BELIZE [ Printed by the Government Printer,] No. 1 Power Lane, Belmopan, by the authority of the Government of Belize. REVISED EDITION 2003 Belize Agricultural Health Authority [CAP.211 beginning at "Then add 1 ml potassium thiocyanate solution and mix". Measure the extinctions of the solutions at a wavelength of 4 70 nm and construct a graph relating extinction to the number of micrograms of molybdenum. NOTE: The acidity of the final solution must not exceed 1.5 N with respect to hydrochloric acid; with more strongly acid conditions, fading of the colour will occur. FOURTH SCHEDULE METHODS OF ANALYSIS OF FEEDING STUFFS (A "decimal" system has been adopted for the numbering of divisions and sub-divisions in this Schedule. It is explained at the beginning of the Schedule to these Regulations). The main divisions in this Schedule are as follows: 1. Preparation of the sample for analysis. 2. Determination of moisture. 3. Determination of oil. 4. Determination of protein. 5. Determination of ammoniacal and urea nitrogen. 6. Determination of phosphoric acid. 7. Detennination of fibre. 8. Detem1ination of sugar. 9. Determination of salt. 10. Determination of ash. 11. Detem1ination of calcium. 12. Determinationofcopper. 13. Determination of magnesium. NOTE: References to "water" means purified water as defined in the British Pharmacopoeia. All reagents used should be of analytical quality. 469 THE SUBSIDIARY LAWS OF BELIZE [ Prin.ted by the Governm.ent Printer, ] No. I Power Lane, REVISED EDITION 2003 Belmopan, by the authority of the Government of Belize. 410 CAP. 211] Belize Agricultural Health Authority 1. Preparation of sample for analysis With some materials, fine grinding may lead to loss or gain of moisture, and allowance for this must be made. Grinding should be rapid as possible and unnecessary exposure to the atmosphere avoided. Grinding in a laboratory mill is usually quicker than grinding in a mortar although the latter is permissible. 1.1 If the sample is in a fine condition, mix thoroughly and transfer a portion of not less than 100 g to a non-corrodible container provided with an airtight closure. 1.2 If the sample is not finely ground, mix thoroughly and further grind a portion of not less than 100 g. Transfer the portion so prepared to a non-corrodible container provided with an airtight closure. 1.3 If the sample is appreciably moist or if for any reason the process of grinding and mixing are likely to result in loss or gain of moisture, take a sample immediately after the preliminary mixing procedure described in paragraph 1.2 or the preliminary grinding and mixing procedure described in paragraph 1.3 for the determination of moisture by the method described in paragraph 2. Determine also the moisture content in the finally prepared sample so that the results of the analysis may be corrected to correspond with the sample in its original condition as regards moisture. 2. Determination of Moisture Weigh to the nearest mg about 5 g of the sample, heat at l00'C for 2 to 3 hours, cool in a desiccator and weigh. Reheat for another hour, cool and reweigh. If the difference in weight exceeds 10 mg, continue the heating and cooling procedure until a weight constant within 2 mg is attained. Calculate the total loss of weight as a percentage of the original weight and regard as moisture. THE SUBSIDIARY LAWS OF BELIZE [ Printed by the Government Printer,] No. 1 Power Lane, Belmopan, by the authority of the Government of Belize. REVISED EDITION 2003 Belize Agricultural Health Authority [CAP.211 3. Determination of Oil 3.1 Reagent Light petroleum boiling 40-60°C. 3.2 Procedure 3 .21 For feeding stuffs not containing milk powder and/ or oil or fat fortified milk powder. Weigh to the nearest mg about 3 - 5 g of the sample; transfer to an extraction apparatus and extract with light petroleum for a period of at least four hours. Transfer the residue of the feeding stuff from the extraction apparatus to a small mortar, grind lightly and return it to the extraction apparatus. Wash out the mortar with a small quanitity oflight petroleum and add the washings to the contents of the extraction flask. Continue the extraction for at least another hour. The extract should be clear but if seen to include insoluble matter pour it through a filter paper or cotton wool plug into another weighed flask; wash the extraction flask and filter twice with light petroleum and add the washings to the contents of the second weighed flask. Remove the bulk of the solvent from the flask, dry at 100°C for two hours, cool and weight. Reheat at 100°C for thirty minutes, cool and again weigh. The second weight should not differ by more than 1 or 2 mg from the first weight. Regard this light petroleum extract as oil. Where a sample is presumed to have an oil content in excess of ten percent or where there is reason to believe that the whole of the oil will not be removed from the feeding stuff in a five hour extraction, place a fresh flask on the extraction apparatus and continue the extraction with a fresh quantity oflight petroleum for at least a further hour. Filter and wash into a second weighed flask; dry and weigh as described in the preceding paragraph. 3 .22 For milk powders, including oil or fat fortified milk powders, and feeding stuffs containing milk powder and/or oil or fat fortified milk powder. 471 THE SUBSIDIARY LAWS OF BELIZE [ Prin.ted by the Governm.ent Printer, ] No. I Power Lane, REVISED EDITION 2003 Belmopan, by the authority of the Government of Belize. 472 CAP. 211] Belize Agricultural Health Authority 3.221 Reagents Ammonia solution ( d = 0. 9 1 ). Diethyl ether, peroxide free. Ethanol, 95% v/v. Light petroleum, boiling range 40 - 60°C. 3.222 Procedure Weigh, to the nearest 0 .2 mg, 1 - 1.1 g of the feeding stuff and transfer to a fat extraction tube provided with a glass stopper and siphon tube. Add 9 ml water, temperature 60 - 70°C, stopper the tube and shake vigorously until the sample is uniformly suspended. Cool to room temperature, add 1.5 ml ammonia solution, stopper and shake thoroughly. Add 10 ml ethanol, using some to rinse the stopper and collect the washings in the extraction tube. Stopper the tube and shake thoroughly. Add 25 ml diethyl ether, using some to wash the stopper as before, stopper the tube and shake vigorously for ninety seconds. Cool the tube and remove the stopper cautiously so as to avoid loss of contents. Add 25 ml light petroleum, washing the stopper as before, stopper the tube and shake vigorously for ninety seconds. Allow to stand for fifteen minutes, or until the solvent layer separates cleanly. Remove the stopper, insert a tube and transfer the ethereal layer to a flask. Raise the siphon and, before removing it from the tube, wash it down with 15 ml of disthyl ether. Remove the siphon tube and rinse the tip with ether, collecting the rinsings in the flask. Add 1 ml ethanol to the tube, stopper, shake vigorously for ninety seconds, cool, remove the stopper, add 15 ml light petroleum and again shake for ninety seconds. Allow to stand for fifteen minutes or until the layer separates cleanly, fit the siphon tube and remove the solvent layer to the flask as before. Carry out a third extraction with 15 ml diethyl ether followedby 15 ml light petroleum in the same way, collecting the solvent in the flask. Remove the THE SUBSIDIARY LAWS OF BELIZE [ Printed by the Government Printer,] No. 1 Power Lane, Belmopan, by the authority of the Government of Belize. REVISED EDITION 2003 Belize Agricultural Health Authority [CAP.211 solvent from the flask by evaporating and dry the flask lying on its side at 100°C for two hours; cool in a desiccator and weight. Reheat at 100°C for thirty minutes, cool and weigh. Add about 20 ml light petroleum to the flask and swirl gently to dissolve the oil, warming if necessary. Allow any residue to settle, then decant the supernatant solution taking care to retain any insoluble residue. Add another 20 ml light petroleum, swirl cautiously and decant as before. Repeat with further small quantities oflight petroleum until all the oil has been removed from the flask. Reheat the flask, lying on its side, at 100°C for one hour, allow to cool and weigh. Record the difference in weights as the weight of oil. 4. Determination of Protein Ascertain the percentage of nitrogen by the method described in paragraph 4.3, and calculate the percentage of protein by multiplying the result by 6 .25. 4.1 Nitrogen 4.2 Reagents Boric acid/indicator solution - add 5 ml of indicator solution (0.1 % methyl red and 0.2% bromocresol green in alcohol) to 1 litre saturated boric acid solution. Sodium hydroxide solution, 50% w/v - dissolve 500 g sodium hydroxide in water and dilute to 1 litre. Sodium sulphate or potassium sulphate - anhydrous. Sulphuric acid, concentrated ( d = 1.84) - nitrogen free. Sulphuric acid, ( or hydrochloric acid), 0.2 N. Paraffin wax. Copper sulphate. 473 THE SUBSIDIARY LAWS OF BELIZE [ Prin.ted by the Governm.ent Printer, ] No. I Power Lane, REVISED EDITION 2003 Belmopan, by the authority of the Government of Belize. 474 CAP. 211] Belize Agricultural Health Authority 4.3 Procedure Weigh to the nearest mg about 2 g of the sample ( or such an amount as shall contain not more than 250 mg nitrogen) and transfer to a kj eldahl flask. Add 25 ml concentrated sulphuric acid, approximately 0.5 g copper sulphate, and 10 g anhydrous sodium sulphate or potassium sulphate. Heat gently over a small flame until ffothing ceases and the liquid is practically colourless. Continue to heat for a further two hours. Avoid local overheating. If frothing is excessive, add about 0.5 g paraffin wax. Dissolve the cooled digest in water, and make up to a total volume of about 250 ml. Taking precautions against loss of ammonia, add sufficient 50% sodium hydroxide solution to neutralise the acid and 10 ml in excess; mix well and connect immediately to a distillation apparatus. Distil into 10 ml of boric acid indicator solution, diluted with 20 ml water, controlling the rate of distillation so that not less than 150 ml distil in thirty minutes. Titrate the distillate against the standard 0.2 N acid solution. Carry out a blank test on the reagents. Express the result in tenns ofnitrogen. 1 ml 0.2 N 0.0028 g nitrogen. NOTE: Where there is reason to suspect that the sample contains nitrogen in the form of ammoniacal, nitrate or urea nitrogen, the appropriate detennination should be made as described in paragraph 3 .2, 3 .4 of the Third Schedule or paragraphs 5 .1, 5 .2 of this Schedule and the amount so obtained deducted from the total nitrogen content. In the case of compound feeds containing urea, the deduction of the nitrogen content is unnecessary for the calculation of the protein content. 5.1 Determination of ammoniacal nitrogen Weigh to the nearest mg about 5 g of the sample, transfer to a 250 ml volumetric flask, add 200 ml water and shake well to ensure solution of all water soluble matter. Dilute to 250 ml, filter and transfer 50 ml of the filtrate to a distillation flask. Add about 300 ml water and 20 ml 50% sodium hydroxide solution. THE SUBSIDIARY LAWS OF BELIZE [ Printed by the Government Printer,] No. 1 Power Lane, Belmopan, by the authority of the Government of Belize. REVISED EDITION 2003 Belize Agricultural Health Authority [CAP.211 Distil into 10 ml boric acid indicator solution, diluted with 20 ml water, controlling rate of distillation so that not less than 150 mi distil in thirty minutes. Titrate the distillate against the standard 0.2N acid solution. Express the result in terms of ammonia nitrogen. 1 ml O .2N acid - 0. 0028 g nitrogen. 5.2 Determination of urea nitrogen 5.21 Reagents Activated charcoal. Carrez solution 1 - dissolve 21.9 g zinc acetate dihydrate in water and 3 ml glacial acetic acid and dilute to 100 ml with water. Carrez solution 2 - dissolve 10.6 g potassium ferrocyanide in water and dilute to 100 ml. 4-Dimethylaminobenzaldehyde solution - dissolve a 2g 4-dimethylaminobenzaldehyde in 10 ml concentrated hydrochloric acid and dilute to 100 ml with propan-2-ol. Hydrochloric acid, 0.01 N. Sodium acetate solution - dissolve 136 g sodium acetate ( CH 3 COON a .3H 2 0) in water and dilute to 1 litre. Urea standard solution - dissolve 1 g urea in water and dilute to 100 ml. 5.22 Procedure Weigh to the nearest mg about 5 g of the sample ( or such an amount as shall contain not more than 250 mg urea) and transfer to a 250 ml volumetric flask. Add 150 ml 0.02 N hydrochloric acid, shake for thirty minutes then add 10 ml sodium acetate solution and mix well. Add 1 g activated charcoal ( see note) to the flask and shake well, and stand for a further fifteen minutes. Add 5 ml Carrez solution 1, followed by 5 ml Carrez solution 2, mixing well between 475 THE SUBSIDIARY LAWS OF BELIZE [ Prin.ted by the Governm.ent Printer, ] No. I Power Lane, REVISED EDITION 2003 Belmopan, by the authority of the Government of Belize. 476 CAP. 211] Belize Agricultural Health Authority additions. Dilute the volume with water and mix well. Filter a portion through a suitable dry filter paper into a clean dry 250 ml beaker. Transfer a 10 ml aliquot of the filtrate to a 50 ml flask, add 10.0 ml 4-dimethylaminobenzaldehyde solution, dilute to 50 ml with water, mix well and allow to stand for ten minutes. Determine the extinction of the solution at 435 nm using a 1 cm cell against a blank of 10 ml 4-dimethylaminobenzaldehyde reagent diluted to 50 ml with water. Calculate the urea content of the sample by reference to a calibration graph prepared at the same time as the test sample. (mg urea x 0.4665 = mg urea nitrogen). Establish the calibration graph as follows: Measure amounts of standard urea solution corresponding to 50, 100, 150, 200 and 250 mg of urea into a series of250 ml volumetric flasks and proceed as described above commencing at "Add 150 ml 0.02 N hydrochloric acid .... ". Measure the extinctions of the solution, and construct a graph relating the extinctions to the milligrams of urea. NOTE: If the sample is highly coloured due to the presence of molasses the proportion of activated charcoal must be increased to 5 g. The final solution after filtering should be colourless. 6. Determination of Phosphoric Acid For the purposes of Act Part IV "phosphoric acid" means P 2 0 5 (molecular weight 142.04). Phosphoric acid shall be determined by the spectrophotometric ( vanadium phosphomolybdate) method. 6.1 Spectrophotometric (Vanadium Phosphomolybdate Method) 6.11 Reagents Calcium oxide - finely ground. THE SUBSIDIARY LAWS OF BELIZE [ Printed by the Government Printer,] No. 1 Power Lane, Belmopan, by the authority of the Government of Belize. REVISED EDITION 2003 Belize Agricultural Health Authority Hydrochloric acid, concentrated ( d = 1.18) Nitric acid, contentrated ( d = 1. 4 2). [CAP.211 Potassium dihydrogen phosphate solution ( stock phosphate solution) -dissolve in water 1.917 g potassium dihydrogen phosphate previously dried at 105°C for one hour and dilute to 1 litre. Potassium dihydrogen phosphate ( standard phospate solution) - dilute 50 ml stock solution to 250 ml with water 1 ml= 0.2 mg phosphoric acid (P20s). Vanado-molybdate reagent - dissolve separately 20 g ammonium molybdate and 1 g ammonium vanadate in water, mix, acidify with 140 ml concentrated nitric acid and dilute to 1 litre. 6.12 Dissolution of the sample Weigh to the nearest mg about 5 g of the sample into a capsule or dish; and 1 g calcium oxide, mix well and thoroughly wet with a little water. Dry the mixture and incinerate at a temperature not exceeding 500°C until completely charred. Cool, transfer the contents of the capsule or dish to a 250 ml beaker and add 10 ml water; then add slowly 12 ml concentrated hydrochloric acid, taking suitable precautions to avoid loss by effervescence, and finally 5 ml of concentrated nitric acid. Heat to incipient boiling and keep at this temperature for ten minutes. Dilute with about 10 ml of water, filter, transfer the insoluble matter to the filter paper with a minimum amount of water and wash twice with small volumes of water. Then transfer the filter paper and insoluble matter to the original capsule or dish and incinerate until all the carbon is destroyed. Combine the ash with the filtrate and heat to boiling point. Cool, transfer to 250 ml volumetric flask, dilute to the mark, mix well and filter. Discard the first 10 or 20 ml of the filtrate. 477 THE SUBSIDIARY LAWS OF BELIZE [ Prin.ted by the Governm.ent Printer, ] No. I Power Lane, REVISED EDITION 2003 Belmopan, by the authority of the Government of Belize. 478 CAP. 211] Belize Agricultural Health Authority 6.13 Procedure 6.131 Standardisation ofinstrument From a burette measure into a series of 100 ml volumetric flask 25.0, 26.0, 27.0, 28.0, 29.0, 30.0 and 31.0 ml of the standard phosphate solution (i.e. 5.0, 5.2, 5.4, 5.6, 5.8, 6.0, and 6.2 ing phosphoric acid). Add 25 ml of the vanado-molybdate reagent to each flask and dilute to 100 ml with water making sure that the temperature of the reagent and the dilution water is 20°C. Shake and allow to stand for ten minutes. Set the spectrophotometer to the correct wavelength, circa420nm, fill two 1 cm cell with the 5.0 mg solution and check the extinction of the cells. Ifthere is a small difference, select the cell with the smaller reading as the standard reference cell. Determine the apparent extinction at 20°C ( corrected for cell differences) of the 5.2, 5.4, 5.6, 5.8, 6.0 and 6.2 mg phosphoric acid solution referred to the 5 .0, mg. phosphoric acid solution as standard. Plot a calibration graph of scale readings against known phosphoric acid content. 6.132 Analysis of sample Successively dilute a portion of the solution prepared according to paragraph 6.12 so that the final volume of about25 ml contains between 5.5 and6.2 mg phosphoric acid, taking care that the dilution water is at a temperature of 20°C. Transfer this final volume to a 100 ml volumetric flask, add 25 ml of the vanado-molybdate reagent (at a temperature of20°C), dilute to the mark, mix, and allow to stand for ten minutes. At the same time transfer 25 ml of the standard phosphate solution ( at 20°C) into a second 100 ml volumetric flask. Add 25 ml of the vanado-molybdate reagent ( at 20°C), dilute to the mark, mix THE SUBSIDIARY LAWS OF BELIZE [ Printed by the Government Printer,] No. 1 Power Lane, Belmopan, by the authority of the Government of Belize. REVISED EDITION 2003 Belize Agricultural Health Authority [CAP.211 and allow to stand for ten minutes. Measure the difference in extinction at 20°C between the two solutions and estimate the phosphoric acid content of the volume of the unknown solution from the calibration graph. Calculate the phosphoric acid content of the sample from known dilution factors and the weight of the sample. NOTE: Prepare a fresh reference standard for each series of readings on the instrument. 7. Determination of Fibre 7. 1 Reagents Alcohol - industrial methylated spirit. Diethyl ether. Hydrochloric acid, 1 % v /v - dilute 10 ml concentrated hydrochloric acid with waterto 1 litre. Light petroleum - boiling point 40 - 60°C. Sodium hydroxide, 0.313 N - this solution must be free from sodium carbonate. Sulphuric acid, 0.255 N. 7 .2 Procedure Weigh to the nearest mg about 2. 7 to 3. 0 g of the sample, transfer to an extraction apparatus and extract with light petroleum. Alternatively, extract with light petroleum stirring, settling and decanting three times.Air dry the extracted sample and transfer to a dry 1,000 ml conical flask(see note).Add200 ml 0.2555 N sulphuric acid measured at ordinary temperature and brought to boiling point, the first 30 or 40 ml being used to disperse the sample, and heat to boiling point within 1 minute. An appropriate amount of anti-foaming agent may be added if necessary. Boil gently for exactly thirty minutes, maintaining a constant volume 479 THE SUBSIDIARY LAWS OF BELIZE [ Prin.ted by the Governm.ent Printer, ] No. I Power Lane, REVISED EDITION 2003 Belmopan, by the authority of the Government of Belize. 480 CAP. 211] Belize Agricultural Health Authority and rotating the flask every few minutes in order to mix the contents and remove particles from the sides. Meantime, prepare a Buchner fpnnel fitted with a perforated plate by adjusting a piece of cut cotton cloth or filter paper to cover the holes in the plate so as to serve as a support for a circular piece of suitable filter paper. Pour boiling water into the funnel, allow to remain until the funnel is hot and then drain by applying suction. Care should be taken to ensure that the filter paper used is of such quality that it does not release any paper fibre during this and subsequent washings. At the end of the thirty minutes boiling period, allow the acid mixture to stand for one minute and then pour immediately into a shallow layer of hot water under gentle suction on the prepared funnel. Adjust the suction so that the filtration of the bulk of the 200 ml is completed within ten minutes. Repeat the determination if this time is exceeded. Wash the insoluble matter with boiling water until the washings are free from acid; then wash back into the original flask by means of a wash bottle containing 200 ml 0.313 N sodium hydroxide solution measured at ordinary temperature and brought to boiling point. Boil for thirty minutes with the same precautions as those used in the earlier boiling and treatment. Allow to stand for one minute and then filter immediately through a suitable filter paper. Transfer the whole of the insoluble material to the filter paper by means of boiling water, wash first with boiling water then with 1 % hydrochloric acid, and finally with boiling wateruntil free from acid. Then wa-;h twice with alcohol and three times with ether. Transfer the insoluble matter to a dried weighed ashless filter paper and dry at 100°C to a constant weight. Incinerate the paper and contents to an ash at a dull red heat. Subtract the weight of the ash from the increase of weight on a paper due to the insoluble material, and report the difference as fibre. NOTE: In the event of the sample containing 3% or more of calcium carbonate ( chalk or limestone flour), it will be necessary to remove the calcium carbonate before digesting the sample with acid. This can be done at the stage in the THE SUBSIDIARY LAWS OF BELIZE [ Printed by the Government Printer,] No. 1 Power Lane, Belmopan, by the authority of the Government of Belize. REVISED EDITION 2003 Belize Agricultural Health Authority [CAP.211 procedure when the portion taken for analysis has been extracted with light petroleum. The original weight taken for the determination should be such that the actual amount of feeding stuff free from calcium carbonate is between 2. 7 and3.0. Transfer the air-dried extracted sample to a 1,000 ml conical flask, add a quantity of 196 hydrochloric acid more than sufficient to neutralise the calcium carbonate present and stir well. Allow to settle, decant off the supernatant liquid through a filter and wash the residue twice by decantation with water, passing the washings through the filter.Allow the residue and the filterto drain thoroughly. Bring 200 ml 0.255 N sulphuric acid (measured at ordinary temperature) to boiling point and use a portion of this to wash any particles on the filter back into the flask. Add the remainder of the acid to the flask and heat to boiling point within one minute. Continue the determination as described in paragraph 7.2. 8. Determination of Sugar For the purposes of the Act, Part IV, "sugar" means total reducing sugars after inversion expressed as sucrose. Declarations of sugar are required only in respect of molasses, treacle molasses feeds and molassed beet pulp. It is necessary, therefore, as the first procedure, to "clean" the sugar from impurities, or from its absorbent body. The total reducing sugar content is then determined after inversion of the sucrose. 8.1 Reagents F chling 's solution - mix equal volumes of a solution of copper sulphate and a solution of sodium potassium tartrate prepared as follows: Copper sulphate solution- dissolve 69.28 g copper sulphate (CuS0 4 .5H 2 0) in water and dilute to l litre. Sodium potassium tartrate solution - dissolve 346 g sodium potassium tartrate 481 THE SUBSIDIARY LAWS OF BELIZE [ Prin.ted by the Governm.ent Printer, ] No. I Power Lane, REVISED EDITION 2003 Belmopan, by the authority of the Government of Belize. 482 CAP. 211] Belize Agricultural Health Authority and 100 g sodium hydroxide in water and dilute to 1 litre. NOTE: The strength of the Fehling's solution should be such that 10 ml is equivalent to 0.0525 g invert sugar. It should be checked by titrating with a solution of pure sucrose ( inverted by the procedure described in the Note following paragraph 8.223) using the procedure described in paragraph 8.223. Hydrochloric acid, N. Methylene blue solution - dissolve 2.5 g methylene blue in water and dilute to 250ml. Phenolphthalein indicator solution- dissolve 0.25 gphenolpthalein in 150 ml industrial methylated spirit and dilute with water to 250 ml. Potassium ferrocyanide solution - dissolve 106 g potassium ferrocyanide in water and dilute to 1 litre. Potassium oxalate solution - dissolve 50 g potassium oxalate in water and dilute to 1 litre. Sodium hydroxide, 10% w/v - dissolve 100 g sodium hydroxide in water and dilute to 1 litre. Zinc acetate solution - dissolve 219 g zinc acetate and 3 0 ml glacial acetic acid in water and dilute to l litre. 8.2 Procedure 8.21 Preparation of the sample 8.211 When the substance is in solid form Weigh to the nearest centigram about 10 g of the sample or a sufficient quantity THE SUBSIDIARY LAWS OF BELIZE [ Printed by the Government Printer,] No. 1 Power Lane, Belmopan, by the authority of the Government of Belize. REVISED EDITION 2003 Belize Agricultural Health Authority [CAP.211 to contain about 2 g sugar. Grind in a mortar with hot water ( temperature not to exceed 60°C) and transfer to a 500 ml volumetric flask using in all about 400 ml water. Shake the flask at intervals during thirty minutes. Add 5 ml potassium Qxalate solution to the contents of the flask, followed by 5 ml zinc acetate solution; mix well and then add 5 ml potassium ferrocyanide solution, make up with water to 500 ml at the correct temperature, mix well and filter. Determine the sugar in 100 ml of the filtrate by the method described in paragraph 8.22. 8.212 When the substance is in liquid form Weigh to the nearest mg about 5 g of the sample and wash with water into a 250 ml volumetric flask using about 200 ml water. To clear the solution add 5 ml zinc acetate solution. Mix, then add 5 ml potassium ferrocyanide solution, again mix, dilute to 250 ml, mix and filter. Determine the sugar in 25 ml of the filtrate by the method described in paragraph 8.22. 8.22 Determination of the sugar content 8.221. Transfer the measured volume of filtrate obtained as described in paragraph 8.211 or paragraph 8.212 to a 300 ml beaker, add 15 ml N hydrochloric acid, dilute to 150 ml with water, cover with a watch glass and heat to boiling point. Continue to boil for two minutes, cool, add two or three drops of phenolphthalein indicator solution,just neutralise with 10% sodium hydroxide solution, transfer to a 200 ml volumetric flask and dilute to 200 ml. Filter ifnecessaty. 8.222 Preliminary Estimation (This estimation is usually necessaty where the percentage of sugar is unknown.) Transfer exactly 10 ml Fehling 's solution to a 250 ml conical flask and add 20 ml water. Add from a burette approximately 10 ml of the filtrate prepared as described in paragraph 8.221, heat to boiling point and boil briskly for one minute. Add three drops of methylene blue solution and titrate from the burette at the rate of 1 ml per fifteen seconds until the blue colour is discharged, the 483 THE SUBSIDIARY LAWS OF BELIZE [ Prin.ted by the Governm.ent Printer, ] No. I Power Lane, REVISED EDITION 2003 Belmopan, by the authority of the Government of Belize. 484 CAP. 211] Belize Agricultural Health Authority contents of the flask being kept boiling throughout the titration. Note the total number of ml required and call this X ml. This titration should not be outside the range of 15 - 40 ml otherwise the determination should be repeated using a more appropriate volume of the filtrate. 8.223 Exact Determination To a 10 ml Fehling's solution in a 250 ml conical flask add from a burette (X- 1) ml of the filtrate prepared as described in paragraph 8.221, together with sufficient water to make a total volume of 60 ml. Heat to boiling point, boil briskly for one and a half minutes and add three drops of methylene blue solution. Titrate from the burette at the rate of approximately O .25 ml per fifteen seconds until the blue colour is discharged, the contents of the flask being kept boiling briskly throughout the titration which must not take more than one and a half minutes. Then the total number of ml used in the determination equals the sugar equivalent of 10 ml Feliling's solution. 10 mlFehling's solution 0.0525 g invert sugar. Not more than 1 ml of filtrate should be required for the completion of the titration. If more than 1 ml is required, then the determination should be repeated using a more closely calculated volume of filtrate for the original addition. The time taken from the initial boiling point until the end of the titration should be about three minutes. If this time is exceeded by more than about twenty seconds, the titration should be repeated. The total copper reducing power should be calculated as invert sugar and multiplied by 0.905 give sucrose. NOTE: The F ehling's solution may be standardised as follows: Dissolve 2.375 g sucrose ( dried at 100°C) in about 100 ml water in a 300 ml beaker, add 15 mlN hydrochloric acidandsufficientwaterto give a volume of 150 ml. Heat to boiling point, boil for two minutes, cool, add two or three THE SUBSIDIARY LAWS OF BELIZE [ Printed by the Government Printer,] No. 1 Power Lane, Belmopan, by the authority of the Government of Belize. REVISED EDITION 2003 Belize Agricultural Health Authority [CAP.211 drops of phenolphthalein solution, just neutralise with 10% soldium hydroxide solution, transfer to a 500 ml volumetric flask and dilute to 500 ml. Then follow the procedure described in paragraph 8.223. 1 ml of this solution - 0.00475 g sucrose 0.005 g invert sugar, i.e., 10 ml Fehling's solution= 10.5 ml of this standard invert sugar solution. 9. Determination of Salt 9.1 Reagent Calcium oxide - finely ground- this reagent must be free from chloride. 9.2 Procedure Weigh to the nearest mg about 5 g of the sample, mix with 1 g calcium oxide and wet with water to a thick paste. Dry the mixture, grind to a fine powder and heat to a tem1perature not exceeding 500°C until all the organic matter has been thorough charred. Extract the residue with repeated portions of hot water, filter, cool the filtrate and dilute to 250 ml in a volumetric flask. Determine the chloride in an aliquot part of the filtrate and express the result in terms of soldium chloride (NaCl). 10. Determination of Ash Weigh to the nearest mg from 2 to 5 g of the sample, incinerate at a temperature not exceeding 500°C until the carbon has been destroyed. Cool, weigh and regard as ash. 11. Determination of Calcium Calcium may be determined by the oxalate method or, alternatively, by the atomic absorption spectrophotometric method. 485 THE SUBSIDIARY LAWS OF BELIZE [ Prin.ted by the Governm.ent Printer, ] No. I Power Lane, REVISED EDITION 2003 Belmopan, by the authority of the Government of Belize. 486 CAP. 211] Belize Agricultural Health Authority 11.1 Oxalate Method 11.11 Reagents Ammonia solution, 2% v/v dilute 25 ml concentrated ammonia solution ( d = 0.91)withwaterto I litre. Ammonium acetate solution - dissolve 500 g ammonium acetate in 500 ml water. Ammonium oxalate solution - saturated aqueous solution. Bromocresol green indicator solution- dissolve 0.05 g bromocresol green in 20 ml ethanol and dilute with water to 100 ml. Citric acid - monohydrate. Hydrochloric acid, 50% v/v - dilute 50 ml concentrated hydrochloric acid ( d 1.18) with water to 100 ml. Potassium permanganate, 0 .1 N. Sulphuric acid, 20% v/v - cautiously add 100 ml concentrated sulphuric acid ( d- 1.84) to 400 ml water, and, while hot, add 0.1 N potassium permanganate drop by drop until a faint pink colour persists. 11.12 Dissolution of the sample Weigh to the nearest mg, about 5 g of the sample into a platinum or silica basin and incinerate at a temperature not exceeding 500°C lllltil all the organic matter has been destroyed. Allow to cool moisten the ash with water and cautiously add 10 ml 50% v/v hydrochloric acid, avoiding loss by use of a cover glass. Wash the cover glass with water, adding the washings to the basin and evaporate THE SUBSIDIARY LAWS OF BELIZE [ Printed by the Government Printer,] No. 1 Power Lane, Belmopan, by the authority of the Government of Belize. REVISED EDITION 2003 Belize Agricultural Health Authority [CAP.211 to dryness. Continue heating for at least one hour to dehydratd any silica which may be present. Cool, add 20 ml water and 10 ml 50% v/v hydrochloric acid, bring to the boil and filter into a 250 ml volumetric flask. Wash the basin the filter with hot water collecting the washings in the flask. Cool, make up to volume andmix. 11.13 Procedure Transfer an aliquot of the filtrate, containing about 40 mg Ca, to a 400 ml beaker and add water to make the volume approximately 150 ml. Add sufficient bromocresol green indicator, 1 -2 g citric acid, and ammonium acetate solution drop by drop until the colour changes to yellow-green (pH 4.0). Bring the solution to the boil and while boiling, slowly add with stirring 20 ml boiling ammonium oxalate solution. Digest the mixture at boiling point for fifteen minutes, allow to cool and stand for at least four hours. Decant the supernatant liquid through a sintered glass crucible (porosity 4). Wash down the sides of the beaker with hot water, stir up the calcium oxalate precipitate and allow to settle. Decant the supernatant liquid through the sintered glass cmcible. Transfer the precipitate to the sintered glass cmcible with 2 % v/v ammonia solution and wash the beaker and cmcible with 2% v/v ammonia solution until the washings are free from chloride. Remove the cmcible and carefully rinse the outside with water, discarding the rinsings. Transfer the bulk of the precipitate to the original beaker and wash the remainder through with hot 20% v/v sulphuric acid, adding the washings to the beaker. Add 70 - 80 ml boiling water and mix to dissolve the precipitate. Heat the contents to 7 5 80°C and titrate with 0 .1 N potassium pem1anganate until a faint pink colour persists for thirty seconds, transferring the cmcible to the beaker towards the end of the titration. 1 ml 0. 1 N KMn0 4 2.0 mg calcium 11.2 Atomic Absorption Spectrophotometric Method 11.21 Apparatus Atomic absorption spectrophotometer. 487 THE SUBSIDIARY LAWS OF BELIZE [ Prin.ted by the Governm.ent Printer, ] No. I Power Lane, REVISED EDITION 2003 Belmopan, by the authority of the Government of Belize. 488 CAP. 211] Belize Agricultural Health Authority Calcium hollow-cathode lamp. 11.22 Reagents Calcium stock solution -dry calcium carbonate at 105°C for one hour. Transfer 2.497 g into a l litre volumetric flask using approximately 100 ml water. Add slowly with swirling 60 ml N hydrochloric acid. When all the calcium carbonate has dissolved, dilute to 1 litre with water. 1 ml= 1 mg calcium. Calcium dilute solution - dilute 20 ml calcium stock solution to 200 ml. 1 ml= 100 ug calcium. Calcium working standard solutions - add 10 ml releasing agent to each of six 100 ml volumetric flasks. Measure 0, 3, 6, 9, 12, 15 ml dilute calcium solution ( 1 ml= 100 ug calcium) into the flasks and dilute to 100 ml with water. The flasks contain 0, 3, 6, 9, 12, 15 ug Ca per ml respectively. Lanthanum oxide solution (releasing agent) - wet 117.3 g lanthanum oxide, La 2 0 3 , low in calcium with water. Add 350 ml concentrated hyhdrochloric acid ( d - 1.18) slowly, and shake until all the lanthanum oxide is dissolved. Allow to cool and dilute to 1 litre with water. 11.23 Procedure Setup the instmmentusingthe line at 422.7 nm. Use a fuel rich flame. Add releasing agent and waferto a suitable aliquot of the sample solution, prepared in accordance with paragraph 11.12 to produce a standard volume of solution to contain between 5 and 10 ug of calcium per ml and 10% v/v releasing agent. Prepare a blank solution from which only the sample has been omitted. Spray water into the frame and zero the instmment. Spray successively, in triplicate, the standard solutions, sample and blank, washing the instrument through with water between each spraying. Plot the mean reading obtained for each standard solution against its calcium content. Determine the calcium content of the sample and blank solutions from the graph and from the difference THE SUBSIDIARY LAWS OF BELIZE [ Printed by the Government Printer,] No. 1 Power Lane, Belmopan, by the authority of the Government of Belize. REVISED EDITION 2003 Belize Agricultural Health Authority [CAP.211 between them calculate the calcium content of the sample. If a number of samples is being examined, one or more standard solutions must be resprayed at intervals during the course of the analyses. 12. Determination of Copper Copper may be determined by the diethyldithiocarbamate spectrophotometric method or alternatively by the atomic absorption spectrophotometric method. 12.1 Diethyldithiocarbamate Spectrophotometric Method 12.11 Reagents Ammonia solution, approximately 6 N - this may be prepared by passing gaseous ammonia into distilled water, or by purifying ammonia solution as described for EDTA-citrate solution below. Carbon trtrachoride, redistilled. Copper sulphate, stock solution- dissolve 0.393 g sulphate, CuS0 4 .5H 2 0, in 100 ml 2 N sulphuric acid and dilute to 1 litre at 20°C with distilled water. Copper sulphate standard solution - dilute 5 ml stock solution to 250 ml with 2 N sulphuric acid at 20°C immediately before use. 1 ml= 2 mg copper. EDTA-citrate solution -dissolve 20 g ammonium citrate and 5 g of the disodium salt of ethylenediamine-tetra-acetic acid (EDTA) in distilled water and dilute to 100 ml. To purify, add 0.1 ml sodium diethyldithiocarbamate solution and extract with carbon tetrachloride. Add a further quantity of sodium diethyldithiocarbamate solution to ensure that it is in excess. Sodium dithyldithiocarbamate solution - dissolve lg sodium diethyldithiocarbamate in distilled water and dilute to 100 ml. Filter the solution if it is not clear. Store the solution in the dark in a refrigerator and discard after 489 THE SUBSIDIARY LAWS OF BELIZE [ Prin.ted by the Governm.ent Printer, ] No. I Power Lane, REVISED EDITION 2003 Belmopan, by the authority of the Government of Belize. 490 CAP. 211] Belize Agricultural Health Authority seven days. Sodiumhydoxide, 0.1 N. Sulphuric acid, 2 N. Thymol blue indicator solution- dissolve 0.1 gthymol blue in2.15 ml 0.1 N sodium hydroxide and dilute to 100 ml with water. 12.12 Preparation of sample Grind the sample to pass a stainless steel sieve having apertures about 1 mm square. With some materials, fine grinding may lead to loss or gain of moisture, and allowance for this must be made. Grinding should be as rapid as possible and unnecessary exposure to the atmosphere avoided. A moisture determination should be carried out on the sample "as received" and again on the sample after grinding, before analysis. 12.13 Dissolution of the sample Weigh to the nearest mg about 10 g of the sample into a silica basin, cover with a silica clock glass, and place in a cool muffle furnace. Raise the temperature to 4 50 + 10°C, and allow to wash overnight; a slow movement of air through the furnace during the initial stages of ashing is desirable. In the case of high-fat materials, care must be taken to avoid ignition of the sample. When all the organic matter has been destroyed, cool, add 10 ml 50% v/v hydrochloric acid, and evaporate to dryness on a water bath. Extract the soluble salts from the residue with two successive 10 ml portions of boiling 2 N hydrochloric acid, decanting the solution each time through the same Whatman No. 451 (or equivalent) filter paper into a50 ml volumetric flask. Then add5 ml 50% hydrochloric acid and about 5 ml 30% v/v nitric acid to the residue in the basin, and take the mixture to dryness on a hot-plate at low heat. Finally, THE SUBSIDIARY LAWS OF BELIZE [ Printed by the Government Printer,] No. 1 Power Lane, Belmopan, by the authority of the Government of Belize. REVISED EDITION 2003 Belize Agricultural Health Authority [CAP.211 add a further 10 ml boiling 2 N hydrochloric acid to the residue and filter solution through the same paper into the flask. Dilute the combined extracts to the mark with distilled water, washing the filter paper in the process. 12.14 Procedure Transfer to a separating funnel a suitable aliquot ( containing not more than 50 ug of copper) of the solution prepared in accordance with paragraph 12 .13. Add 10 ml EDTA-citrate solution, two drops thymol blue indicator solution and ammonia solution until the mixture is coloured green or bluishgreen. Cool the mixture, add 1 ml sodium diethyldithiocarbamate solution and, from a burette, 15 ml carbon tetrachloride. Stopper the funnel, shake vigorously for two minutes and allow the layers to separate. Place a piece of cotton-wool in the stem of the funnel and run off the carbon tetrachloride layer into a dry spectrophotometer cell.Avoid undue exposure of the solution to light. Simultaneously with the test determination, carry out a blank determination on all the reagents used. Measure immediately the extinctions of the test and blank solutions at a wavelength of 436 nm, using carbon tetrachloride in the comparison cell. Read from a previously prepared calibration graph the number of the micrograms of copper corresponding to the observed extinctions of the test and blank solutions, and so obtain by difference the net measure of copper in the sample. Establish the calibration graph as follows: To a series of separating funnels transfer 10 ml ED TA-citrate solution and the following amounts of standard copper solution and 2 N sulphuric acid Copper solution ....... 0 1 2.5 5 10 15 20 25 ml 2 N sulphuric acid ... 25 24 22.5 20 15 10 5 0 ml 491 THE SUBSIDIARY LAWS OF BELIZE [ Prin.ted by the Governm.ent Printer, ] No. I Power Lane, REVISED EDITION 2003 Belmopan, by the authority of the Government of Belize. 492 CAP. 211] Belize Agricultural Health Authority Proceed as for the test solution, as described above, commencing at "two drops thymol blue ..... ". Measure the extinctions of the solutions and construct a graph relating the extinctions to the number of micrograms of copper. 12.2 Atomic Absorption Spectrophotometric Method 12.21 Apparatus Atomic absorption spectrophotometer. Copper hollow-cathode lamp. 12.22 Reagents Copper sulphate standard solution - dissolve 0.393 g copper sulphate, CuSO 4 .5H 2 0, in 0.5 N hydrochloric acid and dilute to 100 ml with 0.5 N hydrochloric acid. 1 ml - 1 mg copper. Dilute this solution as required. Hydrochloric acid, 0.5 N. 12.23 Procedure Set up the instrument using the line at 3 24. 7 nm. Prepare from the standard copper solution a series of solutions, in 0.5 N hydrochloric acid, containing between 0 and 10 ppm copper. Dilute a suitable aliquot of the sample solution, prepared in accordance with paragraph 12.13, with 0.5 N hydrochloric acid to produce a standard volume of solution containing between 0 and 10 ppm copper. Prepare a blank solution from which only the sample has been omitted. Spray distilled water into the flame and zero the instmment. Spray successively, in triplicate, the standard solutions, sample and blank, washing the instrument through with distilled water between each spraying. Record the galvanometer deflection, or the peak height on the recorder if a recording instrument is used. Plot the mean reading obtained for each standard solution against its copper content. Determine the copper content of the sample and blank solutions from THE SUBSIDIARY LAWS OF BELIZE [ Printed by the Government Printer,] No. 1 Power Lane, Belmopan, by the authority of the Government of Belize. REVISED EDITION 2003 Belize Agricultural Health Authority [CAP.211 the graph and from the difference between them calculate the copper content of the sample. If a large number of samples is being examined one or more standard solutions must be resprayed at intervals during the course of the analyses. 13. Determination of Magnesium Magnesium may be determined by the pyrophosphate method or, alternatively, by the atomic absorption spectrophotometric method. 13.1 Pyrophosphate Method 13.11 Reagents Ammonia solution, ( d-0.91 ). Annnonia solution, 5% v/v - dilute 5 ml concentrated annnonia solution ( d 0.91) with water to 100 ml. Ammonium oxalate solution - saturated aqueous solution. Ammonium phosphate solution -dissolve 20 g diammonium hydrogen phosphate, (NH 4 ) 2 HP0 4 , in water and dilute to 100 ml. Calcium wash solution - dissolve 1 g oxalic acid, (COOH) 2 .H 2 0 and 2 g ammonium oxalate in water and dilute to 1,000 ml. Citric acid - monohydrate. Hydrochloric acid, concentrated ( d = 1.18). Hydrochloric acid, 20% v/v - dilute 20 ml concentrated hydrochloric acid ( d 1.18) with water to 100 ml. Methyl red indicator solution -dissolve 0.025 g methyl red in 5 ml 90% industrial 493 THE SUBSIDIARY LAWS OF BELIZE [ Prin.ted by the Governm.ent Printer, ] No. I Power Lane, REVISED EDITION 2003 Belmopan, by the authority of the Government of Belize. 494 CAP. 211] Belize Agricultural Health Authority methylated spirit with the aid of0.5 ml 0.1 N sodium hydroxide. Dilute to 250 ml with 50% industrial methylated spirit. Oxalic acid solution- dissolve 10 g oxalic acid, (COOH\.2Hp, in water and dilute to 100 ml. 13.12 Dissolution of the sample Prepare a solution of the sample as described in paragraph 12.13. 13.13 Procedure Transfer a suitable aliquot ( containing approximately 50 mg magnesium) of the solution, prepared in accordance with paragraph 12.13, to a 500 ml beaker, and add 5% v/v ammonia solution until a slight precipitate is formed. Add citric acid, in small portions, until the precipitate just dissolves, and then 1 gin excess. Heat the solution to 50°C, add 0.2 ml ( four drops) methyl red indicator solution. Neutralise with 5% v/v annnonia solution, and add 1 ml in excess. Add oxalic acid solution until the mixture is just acid, and then 10 ml in excess. Boil the solution for one to two minutes, add 50 ml saturated ammonium oxalate solution, dilute if necessary, to about 200 ml with distilled water, boil for a further minute, and heat on a water bath for at least one hour. Filter through a Whatman No. 40 ( or equivalent) filter paper, wash the residue thoroughly with calcium wash solution. Combine the filtrate and washings, measure the volume, transfer to a beaker and add, while stirring with a glass rod ( avoid touching the sides of the beaker with the rod), 20 ml ammonium phosphate solution. While stirring continuously throughout, neutralise the solution with ammonia solution added drop by drop from a burette and add 20 ml in excess, together with a further 10 ml ammonia solution for each 100 ml of solution in the beaker. Set the beaker aside for at least four hours or, preferably, overnight. Filter through a No. 4 sintered silica crucible and wash the residue with cold THE SUBSIDIARY LAWS OF BELIZE [ Printed by the Government Printer,] No. 1 Power Lane, Belmopan, by the authority of the Government of Belize. REVISED EDITION 2003 Belize Agricultural Health Authority [CAP.211 5% v/v ammonia solution, ensuring that any precipitate adhering to the sides of the beaker and the glass rod is transferred to the crucible. Dry the crucible and residue, transfer to a cool maffle furnace, slowly raise the temperature to 950°C, and heat at this temperature for a half to one hour. Allow the crucible to cool in a dessicator and weigh. Calculate the weight of the precipitate to its equivalent of magnesium by multiplying its weight by 0 .2184. 13.2 Atomic Absorption Spectrophotometric Method 13.21 Apparatus Atomic absorption spectrophotometer. Magnesium hollow-cathode lamp. 13.22 Reagents Hydrochloric acid, 0.5 N. Magnesium sulphate standard solution - dissolve 1.013 g magnesium sulphate, MgS0 4 .7H 2 0, in 0.5 N hydrochloric acid. 1 ml= 1 mg magnesium. Dilute this solution as required. Strontium chloride solution- dissolve 15 g strontium chloride, SrCl 2 6H 2 0, in 0.5 N hydrochloric acid and dilute to 100 ml with 0.5 N hydrochloric acid. 13.23 Procedure Set up the instrument using the line at 285 .2 nm. Prepare from the standard magnesium solution a series of solution, in 0.5 N hydrochloric acid, containing between 0 and 3 ppm magnesium (see note). Dilute a suitable aliquot of the sample solution, prepared in accordance with paragraph 12.13, with 0.5 N hydrochloric acid to produce a standard volume of solution containing between 495 THE SUBSIDIARY LAWS OF BELIZE [ Prin.ted by the Governm.ent Printer, ] No. I Power Lane, REVISED EDITION 2003 Belmopan, by the authority of the Government of Belize. 496 CAP. 211] Belize Agricultural Health Authority 0 and 3 ppm magnesium (see note). Prepare a blank solution from which only the sample has been omitted(seenote). Spray distilled water into the flame and zero the instrument. Spray successively, in triplicate, the standard solutions, sample and blank, washing the instrument through with distilled water between each spraying. Record the galvanometer deflection, or the peak height on the recorder if a recording instrument is used. Plot the mean reading obtained for each standard solutions against its magnesium content. Determine the magnesium content of the sample and blank solutions from the graph and from the difference between them calculate the magnesium content of the sample. If a large number of samples is being examined one or more standard solutions must be resprayed at intervals during the course of the analyses. NOTE: If the sample contains phosphate add strontium chloride solution, at the rate of 5 ml for each 50 ml diluted sample solution, before adjusting to standard. FIFTH SCHEDULE PERMIITEDADDITNESANDPROVISIONSRELATINGTOTHEIRUSE 1. Subject to the following provisions of this Schedule, no material intended for use as a feeding stuff shall contain ( a) any other antioxidant other than an antioxidant of a name and description specified in Part I of the Table in this Schedule nor any added antioxidant of name and description so specified in proportions which, taking account of any such antioxidant which is nom1ally present, exceed 150 parts per million in whole feeding stuffs either separately or in combination with other antioxidants so specified; (b) any added colourant other than a colourant of a name specified in Pali 11 of the Table to this Schedule nor in the case of material intended for use as a feeding stuff for poultry any added colourant of a name specified in section A of the said Part II of the Table to this Schedule in propor THE SUBSIDIARY LAWS OF BELIZE [ Printed by the Government Printer,] No. 1 Power Lane, Belmopan, by the authority of the Government of Belize. REVISED EDITION 2003 Belize Agricultural Health Authority [CAP.211 tions, which, taking account of any such colourant which is naturally present, exceed eighty parts per million in the whole feeding stuff either separately or in combination with other colourants so specified; ( c) any added emulsifier, stabiliser or binder other than an emulsifier, stabiliser or binder of a name or description specified in Part III of the Table to this Schedule; ( d) any added vitamin D2 or vitamin D3, save that material intended for use as a feeding stuff for any animal of a kind specified in the second columnof. (i) Section A Part IV of the Table to this Schedule may contain vitamin D2 or D3 (but not both added) in proportions which taking account of any such vitamin which is naturally present, do not exceed those specified in the third column thereof in relation to kind of animal; (ii) Section B of the said Part IV may contain vitamin D3 in pro portions which taking account of any vitamin D3 which is naturally present, do not exceed those specified in relation thereto in the third column thereof in relation to the kind of animal. ( e) any added substance of a description specified in the first column of Part V of the Table to this Schedule in proportions which, taking account of any such substance which is naturally present, exceed those specified in relation thereto in the second column of the Table; (±) any added copper save that intended for use as a feeding stuff (i) for pigs may contain copper (whether naturally present or added) in proportions not exceeding one hundred parts per million; 497 THE SUBSIDIARY LAWS OF BELIZE [ Prin.ted by the Governm.ent Printer, ] No. I Power Lane, REVISED EDITION 2003 Belmopan, by the authority of the Government of Belize. 498 CAP. 211] Belize Agricultural Health Authority (ii) for any other kind of animal other than pigs or sheep may contain copper (whether naturally present or added) in proportions not exceeding fifty parts per million; (iii) for sheep (whethernatural oradded) not exceedingtwenty five parts per million; (g) any added urea save that intended for use as a feeding stuff for bulls, cows, steers, heifers, calves, sheep or goats may contain urea. 2. If the material is intended for mixing with other materials before use as a feeding stuff and it contains any added substance mentioned in Part I, II, IV or V of the Table to the Schedule or added copper in proportions which, taking account of any substance or copper which is naturally present, do not exceed in each case five times the maximum content specified in relation to the substance in paragraph 2 above or in the Table below, that material may be imported into Belize or sold or processed with a view to sale for use as a feeding stuff if it is marked with the following statement "Feeding Stuffs Regulations. This feeding stuff may only be used for (X) up to a quantity of (Y) grammes per kilogramme." The statement shall be completed by inserting at (X) the kind and, if appropriate the age group of the animal for which the material is intended and at (Y) by inserting such a figure that if the statement is put into effect, the material used as a feeding stuff will comply with the preceding provisions of this Schedule. In this statement they may be substituted for the words "grammes per kilogrannne; the symbol "lb per cwt" or "lb per ton". 3. If the material containing any added iron, iodine, cobalt, manganese, selenium, zinc, vitamins (other than vitamins A, Dor E) or pro-vitamins, in confonnity with the provisions of this Schedule is marked with a statement, additional to the statutory statement required by section 4, containing the name and statement of the total amount present ( whether naturally present or added) if any iron, iodine, cobalt, manganese, selenium, zinc, vitamins ( other than vitamins A, Dor E) or pro-vitamins respectively it shall be marked in a manner specified in section 4. THE SUBSIDIARY LAWS OF BELIZE [ Printed by the Government Printer,] No. 1 Power Lane, Belmopan, by the authority of the Government of Belize. REVISED EDITION 2003 Belize Agricultural Health Authority [CAP.211 4. The drugs mentioned in Part VI to this Schedule shall be permitted drugs for the purposes of this Schedule. TABLE PART! PERMITTED ANTIOXIDANTS Name Octyl gallate .......................... .. Dodecyl gallate ...................... .. N-propyl gallate .................... .. BHA ...................................... . BHT ...................................... . Ethoxyquin ............................. . Chemical Description Octyl 3, 4, 5-trihydroxybenzoate Dodecyl 3, 4, 5-trihydroxybenzoate N-propyl 3, 4, 5-trihydroxybenzoate Mixture of3- and2-tertbutyl 4-hydroxyanisole 2, 6-di (tert-butyl)-4-methylphenol 6-ethyoxy-1,2-dihydroxy-2, 2-4-trimethylquinoline 499 THE SUBSIDIARY LAWS OF BELIZE [ Prin.ted by the Governm.ent Printer, ] No. I Power Lane, REVISED EDITION 2003 Belmopan, by the authority of the Government of Belize. soo CAP. 211] Belize Agricultural Health Authority PART II PERMITTED COLOURANTS Section A Capasanthen ....................................... . Lycopen ............................................. . Beta-8,-apo-carotenal ........................ . Ethyl ester ofbeta-8-apocarotenoic acid Lutein ................................................. . Cryptoxanthin ...................................... . Violaxanthin ........................................ .. Canthaxanthin ...................................... . Zeaxanthin ........................................... . PART III SectionB Patent Blue V Curcumin Amaranth Tartrazine OrangeG Greens Indigo cannine Brilliant Black (Black PN) Cannoisine Ponceau4R Sunset Yellow FCF BrownKF Red6B PEH.MHTEU EMULSlrlEKS, SlAl::HLlZEKS AND JJlNUEKS Lecithin, alginic acid, sodium alginate, potassium alginate, ammonium alginate, calcium alginate, 1,2-dihydroxypropyl alginate, agar-agar, carragen, carob seed flour, tamarind seed flour, guar, guar gum, guar seed flour, gum tragacanth, gum arabic, sorbitol, mannitol, glycerol, pectin, methyl cellulose, carboxymethyl cellulose, hydroxy-propyl-methyl cellulose, ethyl methyl cellulose, sodium, THE SUBSIDIARY LAWS OF BELIZE [ Printed by the Government Printer,] No. 1 Power Lane, Belmopan, by the authority of the Government of Belize. REVISED EDITION 2003 Belize Agricultural Health Authority [CAP.211 potassium or calcium salts of food fatty acids derived from edible oils and fats, or from distilled food fatty acids, mono and di-glycerides of food fatty acids, mono- and di-glycerides of food fatty acids esterified with the following acids: Vitamin acetic, lactic, citric, tartaric and mono-acetyltartaric and dicetyl-tartaric, sucrose esters of food fatty acids, sucro-glycerides of mono- and diglycerides of food fatty acids, polyglycerol esters of the nonpolymerised fatty acids, propylene glycolesters of the food fatty acids, sodium stearoyl-2-lactylate, calcium stearoyl-2-lactylate, lignosulphonates, koalin, bentonites and other montmorillonite c lays, silica, silicates, gelatine, sodium hexametaphosphate, sorbitan esters, polyoxethylene sorbitan esters, disodium ethylenediamine tetraacetate, vermiculite and esters of polyethylene glycol. Kind of Animal SECTION A Pigs Piglets PART IV VITAMINS D Maximum content (International Units per kilogramme in the whole feeding stuff) 2,000 VitaminD2 or VitaminD3 Cattle Calves Sheep 10,000 in milk replacer feeds only 4,000 10,000 in milk replacer feeds only 4,000 Lambs Horses Other kinds except poultry l 0,000 in milk replacer feeds only 4,000 2,000 501 THE SUBSIDIARY LAWS OF BELIZE [ Prin.ted by the Governm.ent Printer, ] No. I Power Lane, REVISED EDITION 2003 Belmopan, by the authority of the Government of Belize. so2 CAP. 211] Belize Agricultural Health Authority VitaminD3 SECTION B Laying hens Poultry other than laying hens 3,000 2,000 Note: In this part of the table "milk replacer feed" means a manufactured feed used as a substitute fornatural milk. PARTY TRACE ELEMENTS Elements JI.Iaximum Contents (Part per million in whole feeding stuff) Iron .............................................. . Iodine ........................................... . Cobalt .......................................... . Manganese ................................... . Zinc ............................................. . Molybdenum ............................... . Selenium ..................................... . THE SUBSIDIARY LAWS OF BELIZE [ Printed by the Government Printer,] No. 1 Power Lane, Belmopan, by the authority of the Government of Belize. 1,250 40 10 250 250 2.5 0.5 REVISED EDITION 2003 Belize Agricultural Health Authority PART VI PERMITTED DRUGS Amprolium Sulphaquinoxaline [CAP.211 NOTE 1: In the case of material of any description specified in the first column of Part II of this Schedule, the Statutory Statement shall contain the particulars, or in the case of any feed supplement the instructions as to handling or use, specified in relation to that material in the second column hereof and also, where there has been added in the course of manufacture or preparation for sale (a) any copper or magnesium, a statement of the total present ( whether naturally present or added) or any copper ( if present in excess of fifty parts per million) ormagnesium(ifpresent in excess of0.5 %); (b) any antioxidant or colourant, either the words "contains permitted antioxidant" or "contains permitted colourant" as appropriate, or the name of the antioxidant or colourant; ( c) any vitamin A, D or E, the name of the vitamin and a statement of the total amount present (whether naturally present or added) and an indication of the period during which that amount will remain present; ( d) any trace element named in Part V of the Fifth Schedule, a statement of the total amount of trace elements present ( whether naturally present or added); any amount referred to (i) in sub-paragraph (a) above shall be expressed as a percentage by weight (unless the amount present is less than 0.1 % by weight in which case it shall be expressed in parts per million); 503 THE SUBSIDIARY LAWS OF BELIZE [ Prin.ted by the Governm.ent Printer, ] No. I Power Lane, REVISED EDITION 2003 Belmopan, by the authority of the Government of Belize. so4 CAP. 211] Belize Agricultural Health Authority (ii) in sub-paragraph (c) above shall be expressedin international units per kilogramme or units per kilogramme; (iii) in sub-paragraph ( d) above shall be expressed in parts per million. SIXTH SCHEDULE MANNER OF TAKING, DIVIDING, MARKING, SEALING AND FASTENING OF SAMPLES Sections 9 (1) and (2) PART I PROVISIONS APPLICABLE TO BOTH FERTILIZERS AND FEEDING STUFFS A. General Provisions 1. In the case of material in any form of package, only unopened packages which appear to the inspector to be the original packages of the material shall be selected for the purpose of the sample. 2. Samples shall not be drawn from any part of the sampled portion which appears to have been damaged. 3. An inspector who proposes to take a sample shall satisfy himself that the conditions in which the material is stored are not such as may cause deterioration of the material and that the material appears not to have been THE SUBSIDIARY LAWS OF BELIZE [ Printed by the Government Printer,] No. 1 Power Lane, Belmopan, by the authority of the Government of Belize. REVISED EDITION 2003 Belize Agricultural Health Authority [CAP.211 contaminated by any other material. 4. In every case the sampling shall be done quickly as possible, consistent with due care, and the material shall not be exposed any longer than is necessary. B. Provisions applicable where the fertilizer or feeding stuff is in solid condition. 5. It shall be assumed that the sampled portion is composed of separate approximately equal parts and the number of such parts is equivalent to ( a) the number of packages to be selected in accordance with paragraph 1 (a) of Part II of this Schedule; or (b) the number of portions, where the sampled portion is in bulk, to be taken in accordance with paragraph 1 (b) of this Schedule. The packages or portions shall be selected on the basis of at least one from each assumed approximately equal part and shall be drawn at random. 6. Where material in packages which an inspector has reasonable cause to believe has been purchased, not for resale in the course of trade but for the purpose of use as a fertilizer or feeding stuff, as the case may be, has been delivered to the purchaser and is to be sampled but some of the consignment is no longer present the number of packages to be selected shall be calculated as if not less than the whole consignment were still present. 7. Notwithstanding anything in these Regulations, a sampling spear shall not be used if objection is raised thereto prior to taking of the sample on the ground that the material is unsuitable. 505 THE SUBSIDIARY LAWS OF BELIZE [ Prin.ted by the Governm.ent Printer, ] No. I Power Lane, REVISED EDITION 2003 Belmopan, by the authority of the Government of Belize. so6 CAP. 211] Belize Agricultural Health Authority 8. Provisions applicable where the fertilizer or feeding stuff is in a liquid or semi-liquid condition (a) ln bottles or containers each not containing more than one quart. The number of bottles or containers to be selected shall be taken at random in accordance with the appropriate scale for solid fertilizers in paragraph 1 (a) of Part 11 of this Schedule. The entire contents of the selected containers shall be emptied into a clean dry vessel and well mixed. From this mixture a sample of between one and two litres shall be drawn. (b) ln containers of more than one litre and not more than 200 litres. The number of containers to be selected shall be taken at random in accordance with the appropriate scale for solid fertilizers in paragraph 1 (a) of Part 11 of this Schedule. The selected eontainers shall be well shaken. An approximately equal portion ofliquid shall then be taken immediately from each of the selected containers and emptied into a clean dry vessel. From this mixture a sample ofbetween one and two litres shall be taken immediately after the mixture has been thoroughly mixed ( c) In a bulk container or containers containing more than 200 litres. (i) If the liquid is homogenous, about one litre shall be drawn from a convenient outlet in the container, into a clean dry vessel, afternmning off sufficient of the liquid to ensure removal of any residues in the outlet. (ii) If the liquid is not homogenous the contents must be stirred thoroughly and the sampling proceed as in sub-paragraph (i). (iii) When the sampled portion consists of two or more containers, THE SUBSIDIARY LAWS OF BELIZE [ Printed by the Government Printer,] No. 1 Power Lane, Belmopan, by the authority of the Government of Belize. REVISED EDITION 2003 Belize Agricultural Health Authority [CAP.211 a sample from each, drawn in the manner described in subparagraph (i) or (ii), as appropriate, shall be placed in a clean dry vessel. After thorough mixing of a sample of about one litre shall be transferred to a clean dry vessel. TABLE Quantities ofliquid or semi-liquid fertilizers or semi-liquid fertilizers and feeding stuffs to be withdrawn in accordance with sub-paragraph ( c) (iii) Where the sampled portion Quantity to be withdrawn does not exceed 5,000 litres does not exceed 50,000 litres exceeds 50,000 litres not less than one litre not less than two litres not less than ten litres PART II PROVISIONS APPLICABLE TO SOLID FERTILIZERS 1. (a) Where the fertilizer is in packages, a numberof packages shall be selected in accordance with the following table: Where the sampled portion consists ofup to three packages ........................................................... . Where the sampled portion consists of more than three but not more the twenty packages .............. . Where the sampled portion consists of more than twenty but not more than sixty packages ............ .. No. ofpackages to be selected for sampling each package 3 4 6 507 THE SUBSIDIARY LAWS OF BELIZE [ Prin.ted by the Governm.ent Printer, ] No. I Power Lane, REVISED EDITION 2003 Belmopan, by the authority of the Government of Belize. so8 CAP. 211] Belize Agricultural Health Authority Where the sampled portion consists of more than No. ofpackages to be selected for sampling each package sixty but not more than one hundred packages... 8 Where the sampled portion consists of more than one hundred but not more than four hundred packages 10 Where the sampled portion consists of more than four hundred packages .. . .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .... .. .. .. 20 Where the number of packages has been selected n accordance with the sub-paragraph either (i) the selected packages shall be emptied separately on a clean surface and well mixed with a shovel and one shovel full taken from each and the shovelsful so taken then be thoroughly mix~ (ii) when the material is of suitable nature, a portion shall be taken from each selected package by means of a closed sampling spear and the separate portions so taken shall be thoroughly mixed From the mixture so obtained, the sample shall be drawn in the following manner Heap the material to form a "cone", flatten the cone and quarter it. Reject two diagonally opposite quarters, mix the remainder and continue quartering and rejection until the remainder is from 500 g to 1,000 gin weight. Alternatively the reduction in size of the gross sample by the quartering method may be effected by the use ofa mechanical device known as a sample divider orrifile. THE SUBSIDIARY LAWS OF BELIZE [ Printed by the Government Printer,] No. 1 Power Lane, Belmopan, by the authority of the Government of Belize. REVISED EDITION 2003 Belize Agricultural Health Authority [CAP.211 (b) In bulk Where the fertilizer is in bulk, a number of portions shall be taken by a shovel or a closed sampling spear as follows: Where the sampled portion does not exceed 100 kg ............................. .. Where the sampled portion exceeds 100 kg but does not exceed 1,000 kg ....................................... . Where the sampled portion exceeds 1,000 kg but does not exceed 10,000 kg ..................................... . Where the sampled portion exceeds 25,000 kg ..................................... . not less than 1 per 25 kg or part thereof not less than 6 not less than 20 not less than 40 The portions, according to whether they have been taken by a shovel or spear, shall be treated in the manner described in paragraph l(a) and the sample drawn in the manner described in that paragraph. Where the fertilizer is in a coarse condition the shovelsful shall be crushed immediately and the final sample after quartering amount of about 1,000 to 1,500 g. Where the fertilizer is bulky or uneven in character special attention must be made to ensure representative sampling. PART III PROVISIONS APPLICABLE TO SOLID FEEDING STUFFS 1. The sample shall be taken in the manner prescribed for a fertilizer in paragraphs 1 (a) or 1 (b) of Part II in this Schedule, where the feedingsmffis in the state of small lumps or meal. 2. Where the feeding stuff is in the form of cake, whether in bags or bulk. 509 THE SUBSIDIARY LAWS OF BELIZE [ Prin.ted by the Governm.ent Printer, ] No. I Power Lane, REVISED EDITION 2003 Belmopan, by the authority of the Government of Belize. s10 CAP. 211] Belize Agricultural Health Authority A number of cakes shall be selected from the different parts of the sampled portion equivalent to the number of portions taken in accordance with paragraph 1 (b) of Part II of this Schedule. The selected cakes shall be broken by a cake breaker or in some other manner so that the whole will pass through a sieve with meshes one and a quarter inch square and then shall be thoroughly mixed. From the mixture so obtained, a sample of not less than 3 kg shall be drawn in the manner described in paragraph 1 (a) of Part II of this Schedule. 3. Where the feeding stuff is in the form of feed blocks or mineral blocks. One block shall be selected irrespective of the size of the sampled portion. From this block a sample of 500 g to 1,000 g shall be taken in any manner. 4. Where the feeding stuff consists of particles grossly differing sizes before the final sample is taken any lumps shall be crushed ( and for this purpose may be separated from other materials) and then the whole thoroughly remixed. From the mixture a sample of500 gto 1,000 gshall be drawn. 5. Where a portion of the feeding stuff is unsuitable for feeding purposes. Where any appreciable portion of the feeding stuff appears to be mouldy, or is otherwise apparently unsuitable for feeding purposes, separate samples shall be drawn of the unsuitable portion and of the residue of the feeding stuff, respectively, and in the case ofunsuitable cakes, the sample may consist of several large pieces representative thereof. PART IV DIVISION, MARKING, SEALING AND FASTENING OF SAMPLE l. Where the sample has been taken in the prescribed manner the person taking the sample shall divide it into three parts, as nearly equal as possible, in the following manner: THE SUBSIDIARY LAWS OF BELIZE [ Printed by the Government Printer,] No. 1 Power Lane, Belmopan, by the authority of the Government of Belize. REVISED EDITION 2003 Belize Agricultural Health Authority [CAP.211 (a) In the case of dry or powered substances: The samples, drawn as prescribed in the foregoing paragraphs, shall be thoroughly mixed on a sheet which will adequately protect the sample from accidental contamination and divided into three approximately equal parts. Each of these parts shall be placed in a clean dry glass or plastic bottle with a close fitting lid so that the original composition of the fertilizer or feeding stuff may be preserved. In the case of materials which undergo change or pick up moisture on exposure to air, the bottle shall have an airtight closure, Each of the said parts shall be so secured and sealed that the bottle containing it cannot be opened without breaking the seal, or alternatively the bottle containing the part of the sample cannot be removed without breaking the seal or the envelope. (b) In the case of substance in a liquid or semi-liquid condition. The sample, drawn as described in the foregoing paragraphs, shall be thoroughly mixed and at once divided into three similar and approxi mately equal parts by pouring successive portions into three glass or plastic bottles with airtight stoppers. 2. Each of the parts shall be sealed and initialled by the person taking the sample. It may also be sealed or initialled by the person on whose premises the sample was taken, or his representative. Each part shall be marked with the name of the material the sampling and some distinguishing reference in such a manner that the particulars so marked can be seen without breaking the seal or seals. 511 THE SUBSIDIARY LAWS OF BELIZE [ Prin.ted by the Governm.ent Printer, ] No. I Power Lane, REVISED EDITION 2003 Belmopan, by the authority of the Government of Belize.