Ministerial Decree No. 2073/87 establishing the method by means of electricity for the detection of cheese adulteration

A8HNA 15 MAPTIOV 1988 TEYXOl: ~EYTEPO APl8MOI: t»YM0V 140 1348 Et»HMEPII THI KYBEPNHIEQI (TEYXOI 4EYTEPO) vnOYPrlKEI AnOCDAIEII & ErKPIIEII Apt0. 2073/87 (2) M i0o6oc; 7IAExi:p0<pOpT)O'T)c; rux 1:1)11 IXVLX)IEUGT) 1108Eicxc; iu otyEAot6tVO yiilot aE i:upui ot'lto 1tpo/3uo yiilot. rENIKO XHMEIO KPATOTI ANOTATO XHMIKO tTMBOTAIO (t1111t6piotaT) 10.11.87) Exovi:otc; U'ltO<jrrj: 1. To iTTpotipo ,:ou fE11txou X71µEiou i:ou Kpiii:o~ 21003/25/ 87. 2. To E6cxipto 6 1:T)c; 1totp. 8 i:ou iip0pou 6 i:ou Noµou 4328/1929 «7tEpi CU0'1:CXO'EWl; ,:OU fElltXOU X71µEiou ,:ou ~pcxi:ouc;•, O'ltWc; i:po1t07tOLTJ 071xE Xotl auµ1tA71pw01jxE µE 1:011 A.N. 754/1937 {otp. 3 7totp. 2 XotL 3) (<I>EK 241/TA/1937). 3. To cxp0po 4 i:ou &toti:iiyµoti:oc; 1:TJ<; 3171c; Oxi:w(3piou 1929 •Ihpi. Xot\10\ltCµou 1:1)<; AEti:oupyicxc; Xotl 1:WII EpjotO'lWII ,:ou Avw,:cx,:ou X71µtxou 1:uµ(3ouAiou• (<I>EK 391/TA/31.10.1929). 4. To Noµo 115/1975 •1t'Epi i:pO'ltO'ltOLTJO'E~ 6tot1:IX~EWII 1:LVWII ,:OU N. 4328./1929» (<I>EK 172/T A/20.8.1975). 5. T7111 ot7tO!p0tCT) 1:WII T1toupyw11 flpoE6pioi{ Xotl Otxo11oµtxw11 ytot otvotµopipwCT) auUoytxwv opy6t:vw11 f11wµo6oi:txijc; xott A1t0<potata,txljc; otpµo6to1:T)'totc; i:ou T1toupyEiou Otxo11oµtxw11 0. 208/181, <I>EK 214/ TB/82. . 6. T7111 T. 1362/ A/5.10.87 ot'ltOipot~ 'tOU flpw8u1toupyou «Kot0opt aµoc; otp1,Lo6tO'tTJ'tWII O''tOII A11ot'ltA7lpW'tTJ T1toupyo OtxovoµtXWII• (<I>EK 535/TB/5. l 0,87), ot1toipotci~ouµE: E yxpi11ouµi. 1:1)11 EipotpµoriJ µE0ooou T)AEX1:pO<pOpT)CT)<; ytot 1:1)11 IXVi XYEUCT) 1:1)<; 1108Eiotc; µE otyEAot6t110 riil~ ,:upLWII ot'ltO 1tpo(3EL0 yiilot, Wl; E~Tjc;: ANIXNETtI:-1 NOE>EIAl: TQN TTPIQN <!>ETA, KE<I>AAOTfPI KAI KAl:EPI AITO ITPOBEIO r AAA ME AfEAAt\INO. f.. K°p1: Tj 1:T)c; µE000ou. fa71pi~E1:otL 0'1:T) 6totcpop6t: 1:T)c; T)AEX1:pocpop71,:1xijc; XL\17l'tlXO't7I-Cotc; 7t0U 1totpoµa16t:~ou11 m 'ltotpot-x-~oc~ETvEc; i:ou otyEAot6wou xott ,ou 1tpo(3Etou r6t: Aotx,oc;. A) flotpcxcxEUTJ 1t71xfiic; 1tohotxpLAotµi611c; 1. A vi:t6pota'tTjptot. ex) TRIS (HYDROXYMETHYL) AMINOMETHANE. (3) Iluxvo u6poxAwptxo o~u (HCL). r) Oupiot. 6) CYANOGUM 41.(95% ACRYLAMIDE + 5%, N, N'- METHY LENBISACRYLAMIDE) E) TEMED (N, N, N: N-' TETRAMETHYLAETHYLENDIA MINE). c,:) T1tEp8ELLXO otµµw11to (xpua'totAALXO). 2. &totAuµcxi:cx. ct) P118µtai:1xo 6tiiluµot 1t71x~ (PH=6, 7). 7,5 'YP· TRIS (lot) XotL 4 ML 'ltUXl/0 HCL (1(3) otpotlWl/01/'totL µE ot'ltE O''totyµevo 11Ep0 µiJ(pt 'tEALXO oyxo 1 LT. (3) &tiiAuµot STOCK ytot 1:1)11 1totpotaXEUTJ i:T)c; 7tTjX'tTjc; 420 'YP· oupicxc; (ly), 60 'YP· CYANOGUM 41 (16) XotL 1 ML TEMED (lE), 6totAUOl/1:otL O'E pu0µt,mxo 6uiAuµot i:71c; 7tTjX'tTjc;"{2ot) µiJ(pL 1:EALXO or)({) 1 LT. To 611i AUµot 6t718EL'totl 6tot 118µou 'totXEiotc; poijc;. 3. T EXlllXTJ 1tcxpcxaXEUTJ 1:T)c; 1t71x,ijc;. Xp71atµo1tOLOUl/1:otL 7tAIXXEc; 6totO''tlXO'E~II 25 CM x 15 CM µE 6uvoti:o 'tT)tot Xot'tCXCXEUTjc; 7tT)X'tTjc; 'lt!Xxouc; 1,5 MM. ftcx xii0E 7tAIXXot ot7totEpW11E 'totl 7t00'01:T)1:CX 70 ML ot'ltO 'tO STOCK 6tiiAuµot 7tTjX'tTjc; (2(3). Me,IX 'tO ,i Aoc; 1:T)c; ot1totipwCT)c; 1tpoa,i8E,ott ci:o 6tiiluµot 32 MG u1tEp8Effxou otµ µwviou. To yiµtcµot 'tWII 7tAotXWII yi11E'totl µE 1tpoaaxiJ ytot 'tT)II ot1toipuriJ EIIO'W µ6t:,wa71c; qiuaaotAi6w11 otipot. 0 1t0AuµEptcµoc; yi11E,ott cE 0Epµoxpotaiot 1tEpt(3illo11,oc;. B) A11i:t6pota,ijptot XotL 6totAuµcx,ot T)AEX'tpoipop71c71c;, cupewa71c; Xotl EJJ. iplXlltO'T)c; 1tpW'tEtl/WII. 1. A11,t6pota'tTjptot. ct) TRIS (HYDROXYMETHYL) AMINOMETHANE. (3) 5,5 6tott0uAo(3otp(3t,ouptxo o~u. r) TptxAwpo~ElXO o~u (T.C.A.). 6) xouµotai1111 ('VOOMA11:IE BPIAAANT BASE). E) ME0otllOAT), 2. &totAuµoti:ot. ct) Pu0µtai:txo 6tiiluµot 7IAEX'tpo6iw11,.,PH 7,4: 5 'YP· 5,5 6tot18uA0(3otp(3t-ro11ptxo o~u (1(3) XotL 1 'YP· TRIS (lot) 6totAU Oll'totl CE ot'ltEO'-Cotyµe110 11Ep0 µEJ(pL 'tEALXO oyxo 1 AT. Pu0µi~EtotL ,:o PH aE 7,4 µE 1tpoa8iJx11 {lot) iJ (1(3). (3) t\tiiluµot ,pLXAW~ELXOU ~ioc; (T.C.A.) 25% ((3iip~/o,xo). r) t\tiiluµot ,ptXAWpo~EIXOU ~Eoc; (T.C.A.) 12,5% ((3iipoc;/o-yxo). 6) &tiiluµot ,ptXAWpo~uxou o~ioc; (T.C.A.) 5% ((30:poc;/oyxo). E) A1iiluµot J(pWO''ttxijc; COOMASSIE BRILLANT BLUE 0,1 % ((30: ~/oyxo): 1 'YP· J(pW0'1:LXTjc; COOMASSIE BRILLANT BLUE, 500 ML µE80t llOA7I, 20 ~L ,p1x).wpo~EIXO o~u 25% (2(3) 6totAUOll'totL O'E ot'ltEO"t0tjµi110 \1Ep0 µiJ(pL 'tEALXOU OjXOII 1 LT. To 6tiiluµot 6tT)0Eii:ott µiaw ,or;xEicxc; poriJc; 6t71871,1xou :x,6t:p,ou. ~> &tiiluµot O~ElXOU o~foc; 10% (oyitoc;/o-yxo). f) ijpoE1:0lµotO'iot 6Eiyµcx,:oc; ,uptou ytot 7IAEX'tpoipop710'T). 1. Avi:t6potc'tTjptot. ct) Atiiluµot oupiotc; BM. (3) 2- MEpXot'lt'tOott8ot110A71. 2. TEx v1xiJ. •uri~O\l"totl 4 'YP· ,uptou, 'tot O'ltOtot 6totAUOl/1:otL O'E 20 ML 6tCXAuµottoc; oupiotc; 8M (lot). To 6tiiAuµot mxpotµEIIEL 'ltEpi1tou 24 H O''tO~ 4 ·c. Axo Aou0Ei ipuyoxi11i:ptO'T) ytot 1:TJY ot1toµiixpuYCT) ,:ou Ai1touc;, a,:u; 8. 000 ai:poipic;/MIN ytot 5 MIN. ME,o: 1:TJY ot1toµiixpu11a71 ,:ou )J,couc;, 1tpoO"ti 8Ev'tott O"tO 6Eiyµot 3-4 ai:otyo11Ec; 2 - µEpXot7t't0ott0ot110A7Jc; (1(3). To 6Eiyµot 1totpotµivEt O'E 0Epµ~potciot 1tEptf3illo11'tOc; ytcx 60 MIN ,:ouMxt ai:ov, 1tptv 1:1)11 T)AExi:poipop7107J. &) HAEx:cpoipop71a71. H TJA!X'tpO<pOpT)CTJ 1tpor:yµoti:01tOLEt1:otl O'E O'UO'X!UTJ xii0E1:1j<; T)AEX'tpoipo P71CTJc; DESAGA, DESAPHOR VA 150 µt ,71 J(p71atµo1toi710'T) .ou pu8 µtO"ttx'ou 6totAUµot,oc; 7IAEX'tpo6iwv (B2ot), O'E O'UYEXTJ XUXAoipopicx, 4'uxo- . µE\IOU O''tOU<; 5 ° C Xotl wuc; 7tOAouc; IXVEO"tpotµµevouc; (µ1Xupo(-) (3icµ01 XIX'tW T)AEX'tpo6to, XOXXlllO ( +) (3iaµcx 7tlXIIW T)AExi:po6t<1},., Ilpw,ot yiYE'totL ot1totipWO'T) ,:ou puOµtO"tLXOU 6totAuµot,oc; (B2ot). Axo Aou0£i 1tpo71Aexi:poqiop71CT) µE ,:u; 1tAo:xEc; 1:TJ<; 7tTjX'tTJ<; :x,wpic; 6ti1 µoti:01, µt O"totbtpiJ 'tlXCT) pEuµoti:oc; 150V ytot µtot wpot XotL O''t7I O'UYEX!LIX Etaiiyo 111:IXl 'tot pEiyµoti:ot O''ttc; uxo6oxic; 1:1)<; 7tAIXXotc; CE µtxpo1t00'01:T)'tE<; ( 1 o ... 50µL).- Koti:07tl\l otXOAOu0d 71 7JAEX1:poipop71c71 CE O''tot0EpTJ ElltCXO'T) 1, 5 MA IXVIX 6Eiyµcx ytot 16 wpEc;. E) t~ptwCT)· xott Eµip0tllLO'T) i:w11 ~wvwv ,:w11 1tcxpo:-x-xotCE"ivwv 1. ti:Epiwc71 ,w11 1tpw,Et11wv. 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E!,MPIX\llO'T) 1:WII 1tplu'tEt\lW\I. 0 ot7tOJ(pWµoti:tdµoc; 1:1)<:; 7tT)X1:TJ<; 07tOU 6EV u1t6t:pxou11 Cw,m; 1tpwtE"i \lWII, yi11Ei:ott µE o!;txo o~u I 0% (B2ai:). fi11ovi:cxt 6tot6o:x,txii ~E7tAuµot,Ot µiJ(pL ,:ou E7tt0uµT)'tOU (3ot0µou ot7tOJ(pWµot'tlO'µou. tT) Mhp71CT) 1:W\I ~W\IW\I 1:1)<; 1totpix-X~XCX~Ei1171i; ME 1:TJ (3oij0Etot EIIO<; µE,p71i:txou xot'totrpot~IXOU opy6t:11ou (SCANNER) µE,pthcxt 71 07t'tlXTJ 7tllX\IOTI)'tlX 1:W\I xpwµot,tcµe11w11 Cw11wv JT)<; 7totpii-x xotCELIIT)<; µE <pihpo µijxouc; xuµoti:oc; 595 NM, 07tO'tE CE Et6txo Xotp,i Xot i:cxyp6t:ipovtotl m cxv,ia,otxEc; xcxµ1tuAEc;, o,ot11 J(p71atµo1totti tott 71 µi0o6o,:; ytot 71µt1tOCO'tlXO 1tpoc6toptcµo. Z) flpoipiA 1t71xi:71c; 1t0Auotxpuhµi611c; µEi:6t: T)AEx,po<pop71071 1t01pii-x xot~E"ivwv otyEAot6t110U xiu 1tpof3EtOU yiiAotxto,:;. EG>HMEPII THI KVBEPNHIEQI (TEVXOI llEVTEPO) a: (-) 1totpci-x-x01{EivEt; 1tccp«-x-x01CtiVE«; 1tpo(iELOU 01y£A016LVOU Il01p01't1jp1jaEu; I. Eciv XP"la4L01tOLTJOEi iliou mou auaxEUTJ TJAEX'tpOtpOP11G11t;, 1tp(! a01p1-10Cov't01L 01viloycc OL n:OaO'tl}'t~ 'tc.>V ccvn6p01a't"1Piwv TLCI 'tl}V n:otpcc CJXEUTj 'tl}t; 1t11X'tTJ«; XCIL yt01 euOfLLG'tLXO 6LCXAUfL01 'tWV ')AEX'tpo6iwv. 1. H fLiOoooi; fL1tOpd 1101 XflTIGLfL01tOLTj0d XCI.L YLCI 'tOV TJf!L1tOGO'tLXO n:poa6mpLGfLO voOEicci; 'tUpL6>V i.iwrElot6LVo ycv.ci, i>anpot om:o ai>yxpLG"I µE oEi1fLot't01 'tUpLWV yvwatl)t; n:tptEX'tLXO'tTJ't«u; a£ y& otyElci&xt;. o Ilpot6poi; o r potfLfLCl'ticu; 1:'ttl.. Xot't{TJjL01WotXOt; Xotp. X011-101Ai611c; T01 1-1U.71: Euotyy. TaLiotpL6cu;. AY't. &01axotAIXXT)t;, Il01V. KW't'tl}t;, EuotTT. 1:u~WV'ljt;, &L~. cJ>p01jXIX't0t;. Eyxpivouf!E 'tl}V n:otpccn:civw 011totpotG11 'tOU Avw'tlX'tOU XTjf!LXOil I:uµ fiouliou xccflwc; xoo 't1} 611µ.oaiEuG"I 'tl}t; G't1}V E'P"lf!Epi&t 't1)t; K u(iEpv71 auu,; x01L opi{oUf!E o'tL Oct otpXLGEL 71 LoXUt; 'tl}t; 0111:0 't1JV 1Jf!ipot 1tou 801 611 µoatEuOEi. AOTjvot, 23 cJlE~pouotpiou 1988 ANAlll\HPQTH[ OIKONOMIKON rEnPr. nETIOI OI YllOYPfOI fEOPllA[ rlAN. noTTAKHI EMnOP10Y NIK. AKPITIAHI 1349